辽宁省营口市长角血蜱携带立克次体和埃立克体调查  

Investigation of Rickettsia and Ehrlichia harbored by Haemaphysalis longicornis in Yingkou City,Liaoning Province,China

作　　者：刘子维[1] 李华锋 LIU Zi-wei;LI Hua-feng(Yingkou Center for Disease Control and Prevention(Yingkou Health Supervision Institute),Yingkou,Liaoning 115000,China)

机构地区：[1]营口市疾病预防控制中心(营口市卫生监督所),辽宁营口115000

出　　处：《中国媒介生物学及控制杂志》2024年第6期714-718,共5页Chinese Journal of Vector Biology and Control

摘　　要：目的初步调查了解我国辽宁省营口市长角血蜱携带立克次体、埃立克体类病原体情况。方法用布旗法从营口市盖州市采集游离蜱138只,经鉴定均为长角血蜱,混样后提取DNA,用立克次体、埃立克体属特异性16S rDNA基因引物使用巢式PCR进行检测,并对阳性样本扩增其gltA、groEL和16S rDNA基因,使用BLASTN和PhyML 3.0软件对所获得序列分别进行遗传与系统进化分析。结果从上述蜱中检出立克次体阳性3份,测序结果显示均为敬信立克次体,其16S rDNA、gltA和groEL基因与已知种同源性分别为100%、100%和99.04%;检出埃立克体阳性4份,测序结果显示其16S rDNA基因与Candidatus Ehrlichia shimanensis、Ehrlichia sp.Yonaguni 206及Candidatus E.pampeana均100%同源,groEL基因与Ehrlichia sp.Yonaguni138同源性为100%,而gltA基因与Uncultured Ehrlichia sp.clone SY130同源性为99.62%(覆盖率56%),与尤因埃立克体同源性为82.85%。结论辽宁省营口市的蜱中存在立克次体和埃立克体的流行,可能存在感染人的风险。Objective To investigate the prevalence of Rickettsia and Ehrlichia harbored by Haemaphysalis longicornis in Yingkou City,Liaoning Province,China.Methods A total of 138 free-living ticks were collected using the drag-flag method in Gaizhou county-level City of Yingkou,Liaoning Province.All the ticks were identified as H.longicornis.The samples were pooled for DNA extraction and screened for genera Rickettsia and Ehrlichia using primers targeting their 16S rDNA genes by nested PCR.Subsequently,the gltA,groEL,and 16S rDNA gene sequences were amplified.All the obtained sequences were subjected to genetic and phylogenetic analyses using BLASTN and PhyML 3.0 softwares,respectively.Results Three pooled samples tested positive for Rickettsia,and the species in all samples were identified as Candidatus Rickettsia jingxinensis by sequencing analysis.The 16S rDNA,gltA,and groEL genes showed 100%,100%,and 99.04%identities to previously reported strains.Moreover,four pooled samples tested positive for Ehrlichia sp.,and 16S rDNA gene sequencing showed 100%identity to Candidatus E.shimanensis,Ehrlichia sp.Yonaguni 206,and Candidatus E.pampeana.Moreover,the groEL gene showed 100%identity to Ehrlichia sp.Yonaguni 138,and the gltA gene showed 99.62%identity(56%coverage)to uncultured Ehrlichia sp.clone SY130 and 82.85%identity to E.ewingii.Conclusion The prevalence of Rickettsia and Ehrlichia in ticks from Yingkou of Liaoning Province suggests the potential risk of human infections.

关 键 词：长角血蜱 敬信立克次体 埃立克体 营口市 

分 类 号：Q939.3[生物学—微生物学] R384.4[医药卫生—医学寄生虫学]