脱细胞肝基质复合丝素蛋白水凝胶支架的制备及表征  

Preparation and Characterization of Decellularized Liver Matrix Composite Silk Fibroin Hydrogel Scaffold

作　　者：雷敏 张苗 尹诗韵 孙中涛 刘心雨 付开秀 牛晓 陈国宝 LEI Min;ZHANG Miao;YIN Shi-yun;SUN Zhong-tao;LIU Xin-yu;FU Kai-xiu;NIU Xiao;CHENGuo-bao(School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400054,China)

机构地区：[1]重庆理工大学药学与生物工程学院,重庆400054

出　　处：《高分子通报》2024年第12期1791-1800,共10页Polymer Bulletin

基　　金：重庆市教委科学技术研究项目(项目号KJQN202301136);大学生创新创业训练计划项目(重庆市级)。

摘　　要：近年来,脱细胞基质在组织工程中展现出巨大应用潜力,但如何改善其力学性能仍然是一个问题。为了探究引入丝素蛋白(silk fibroin, SF)改善脱细胞肝基质(decellularized liver matrix, DLM)力学性能的复合水凝胶支架用于肝组织工程的潜能,对大鼠肝脏进行脱细胞处理后,与SF溶液按等浓度不同体积比例混合交联,制成4组SF/DLM复合支架,SF与DLM溶液的比例分别为100:0、75:25、50:50、25:75,并对支架的形态结构、体外降解性、亲水性、力学性能和细胞相容性等进行了表征。研究结果表明SF能够改善复合支架的力学性能,SF比例的增加使支架的孔隙率和抗压强度提高,支架的降解性能和亲水性能得到提升,结合DLM的促细胞生成功能,该复合支架有望应用于肝修复组织工程。In recent years,decellularized matrix has shown great potential in tissue engineering,but how to improve its mechanical properties is still a problem.In order to explore the potential of using a composite hydrogel scaffold with silk fibroin(SF)to improve the mechanical properties of decellularized liver matrix(DLM)for liver tissue engineering,four groups of SF/DLM composite scaffolds were prepared by cross-linking the rat liver with SF solution at the same concentration and different volume proportions.The proportions of SF to DLM solution were 100:0,75:25,50:50,25:75,respectively.The morphological structure,in vitro degradability,hydrophilicity,mechanical properties and cell compatibility of the scaffolds were characterized.The results showed that SF could improve the mechanical properties of the composite scaffold,the porosity,pressure resistance,degradability and hydrophilicity of the scaffold were all improved.Combined with the cell promoting function of DLM,the composite scaffold is expected to be applied in liver repair tissue engineering.

关 键 词：支架材料 脱细胞基质 丝素蛋白 肝组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]