A single-nucleotide polymorphism in Pv PW1 encodingβ-1,3-glucanase 9 is associated with pod width in Phaseolus vulgaris L.  

作　　者：Kun Xu Jinlong Zhu Hong Zhai Qiang Yang Keqin Zhou Qijian Song Jing Wu Dajun Liu Yanhua Li Zhengjun Xia 

机构地区：[1]State Key Laboratory of Black Soils Conservation and Utilization,Key Laboratory of Soybean Molecular Design Breeding,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin,Heilongjiang 150081,China [2]USDA ARS,Soybean Genome&Improvement Lab,Beltsville 20705,USA [3]Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 10081,China [4]Horticulture Department,College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin,Heilongjiang 150000,China

出　　处：《Journal of Genetics and Genomics》2024年第12期1413-1422,共10页遗传学报（英文版）

基　　金：supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA28070000);the Natural Science Foundation of Heilongjiang Province(TD 2023C005);the National Natural Science Foundation of China(32372029 and 32001506)。

摘　　要：Pod width influences pod size,shape,yield,and consumer preference in snap beans(Phaseolus vulgaris L.).In this study,we map Pv PW1,a quantitative trait locus associated with pod width in snap beans,through genotyping and phenotyping of recombinant plants.We identify Phvul.006G072800,encoding theβ-1,3-glucanase 9 protein,as the causal gene for Pv PW1.The Pv PW1^(G3555)allele is found to positively regulate pod width,as revealed by an association analysis between pod width phenotype and the Pv PW1^(G3555C)genotype across 17 bi-parental F_(2)populations.In total,97.7%of the 133 wide pod accessions carry Pv PW1^(G3555),while 82.1%of the 78 narrow pod accessions carry Pv PW1C3555,indicating strong selection pressure on Pv PW1 during common bean breeding.Re-sequencing data from 59 common bean cultivars identify an 8-bp deletion in the intron linked to Pv PW1C3555,leading to the development of the In Del marker of Pv M436.Genotyping 317 common bean accessions with Pv M436 demonstrated that accessions with Pv M436^(247)and Pv M436^(227)alleles have wider pods compared to those with Pv M436^(219)allele,establishing Pv M436 as a reliable marker for molecular breeding in snap beans.These findings highlight Pv PW1 as a critical gene regulating pod width and underscore the utility of Pv M436 in marker-assisted selection for snap bean breeding.

关 键 词：Common bean Pod width FINE-MAPPING β-1 3-glucanase 9 Marker-assisted selection 

分 类 号：S643.1[农业科学—蔬菜学]