Synchronized lineage tracing of cell membranes and nuclei by dual recombinases and dual fluorescent  

作　　者：Xueying Yang Shun He Xufeng Li Zhihou Guo Haichang Wang Zhuonan Zhang Xin Song Ke Jia Lingjuan He Bin Zhou 

机构地区：[1]School of Life Science,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou,Zhejiang 310024,China [2]School of Life Sciences,Westlake University,Hangzhou,Zhejiang 310030,China [3]New Cornerstone Science Laboratory,State Key Laboratory of Cell Biology,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai 200031,China [4]School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China

出　　处：《Journal of Genetics and Genomics》2024年第12期1474-1484,共11页遗传学报（英文版）

基　　金：supported by the National Key Research&Development Program of China(2021YFA0805100,2023YFA1800700,2019YFA0110403,2019YFA0802000);the National Science Foundation of China(82088101,32370885,92368103,32370897);the Westlake Education Foundation,and the Benyuan Charity Fund,Research Funds of Hangzhou Institute for Advanced Study(2022ZZ01015 and B04006C01600515)。

摘　　要：Genetic lineage tracing has been widely employed to investigate cell lineages and fate.However,conventional reporting systems often label the entire cytoplasm,making it challenging to discern cell boundaries.Additionally,single Cre-lox P recombination systems have limitations in tracing specific cell populations.This study proposes three reporting systems utilizing Cre,Dre,and Dre+Cre mediated recombination.These systems incorporate td Tomato expression on the cell membrane and Phi YFP expression within the nucleus,allowing for clear observation of the nucleus and membrane.The efficacy of these systems is successfully demonstrated by labeling cardiomyocytes and hepatocytes.The potential for dynamic visualization of the cell membrane is showcased using intravital imaging microscopy or threedimensional imaging.Furthermore,by combining this dual recombinase system with the Pro Tracer system,hepatocyte proliferation is traced with enhanced precision.This reporting system holds significant importance for advancing the understanding of cell fate studies in development,homeostasis,and diseases.

关 键 词：Lineage tracing Dual recombinases system CRE-LOXP Dre-rox Cell membrane and nucleus labeling ProTracer system 

分 类 号：Q2-33[生物学—细胞生物学]