Enhancing biofilm formation in the hydrogen-based membrane biofilm reactor through bacterial Acyl-homoserine lactones  

作　　者：Yuchao Chen Kun Dong Yiming Zhang Junjian Zheng Minmin Jiang Dunqiu Wang Xuehong Zhang Xiaowu Huang Lijie Zhou Haixiang Li 

机构地区：[1]College of Environmental Science and Engineering,Guilin University of Technology,Guilin 541006,China [2]College of Chemistry and Environmental Engineering,Shenzhen University,Shenzhen 518060,China [3]College of Life and Environmental Sciences,Guilin University of Electronic Technology,Guilin 541004,China [4]Department of Environmental Science and Engineering,Guangdong Technion-Israel Institute of Technology,Shantou 515063,China [5]Guangdong Provincial Key Laboratory of Materials and Technologies for Energy Conversion,Guangdong Technion-Israel Institute of Technology,Shantou 515063,China

出　　处：《Frontiers of Environmental Science & Engineering》2024年第11期145-157,共13页环境科学与工程前沿（英文）

基　　金：support of this work by the Guangxi Natural Science Foundation(China)(No.2022GXNSFFA035033);the National Natural Science Foundation of China(Grant No.51878197);the Research funds of the Guangxi Key Laboratory of Theory and Technology for Environmental Pollution Control(China)(No.2301Z003).

摘　　要：The slow growth rate of autotrophic bacteria and regulation of biofilm thickness are critical factors that limit the development of a hydrogen-based membrane biofilm reactor(H_(2)-MBfR).The acylhomoserine lactone(AHL)mediated quorum sensing(QS)system is a crucial mechanism regulating biofilm behavior.However,the AHLs that promote biofilm formation in autotrophic denitrification systems and their underlying mechanisms,remain unclear.This study explored the impact of AHLmediated QS signaling molecules on biofilm development in H_(2)-MBfR.This study revealed that C_(14)-HSL and C_(4)-HSL are potential signaling molecules that enhanced biofilm formation in long-term stable operating H_(2)-MBfR.Subsequent short-term experiments with C_(14)-HSL and C_(4)-HSL confirmed their ability to increase bacterial adhesion to carrier surfaces by promoting the production of extracellular polymeric substances(EPS).Functional gene annotation indicated that exogenous C_(14)-HSL and C_(4)-HSL increased the abundance of signal transduction(increased by 0.250%–0.375%),strengthening the inter bacterial QS response while enhancing cell motility(increased by 0.24%and 0.21%,respectively)and biological adhesion(increased by 0.044%and 0.020%,respectively),thereby accelerating the initial bacterial attachment to hollow fiber membranes and facilitating biofilm development.These findings contribute to the understanding of microbial community interactions in H_(2)-MBfRs and provide novel approaches for biofilm management in wastewater treatment systems.

关 键 词：Hydrogen-based membrane biofilm reactor (H2-MBfR) Acyl-homoserine lactones(AHLs) Quorum sensing(QS)Biofilm enhancement 

分 类 号：X703[环境科学与工程—环境工程]