蛇葡萄根提取物对大鼠慢性骨髓炎愈合的影响  

Effect ofradix smpelopsis sinicae extract on healing process of chronic osteomyelitis in rats

作　　者：杨文博[1] 高珊 赵军[1] 刘红喜[1] 王兴盛[1] 毕军伟[1] 周君[1] 陈威[1] 符碧峰 彭冉东 王玉磊 YANG Wen-bo;GAO Shan;ZHAO Jun;LIU Hong-xi;WANG Xing-sheng;BI Jun-wei;ZHOUJun;CHEN Wei;FU Bi-feng;PENG Ran-dong;WANG Yu-lei(Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou,Gansu 730050,China;The First People's Hospital of Lanzhou,Lan-zhou Gansu 730050,China;The First Affiliated Hospital,Tianjin University of Traditional Chinese Medicine,Tianjin 300000,China)

机构地区：[1]甘肃省中医院,甘肃兰州730050 [2]兰州市第一人民医院,甘肃兰州730050 [3]天津中医药大学第一附属医院,天津300000

出　　处：《中国矫形外科杂志》2024年第23期2171-2177,共7页Orthopedic Journal of China

基　　金：甘肃省级科技计划项目(编号:21JR11RA207;23JRRA1242);兰州市人才创新创业项目(编号:2021-RC-132);甘肃省卫生健康行业科研计划项目(编号:GSWSKY2021-038);甘肃省省中医药管理项目(编号:GZKP-2023-5)。

摘　　要：【目的】探讨蛇葡萄根提取物对慢性骨髓炎反应的影响。【方法】将大鼠分为5组,分别为正常组、模型组、低剂量、中剂量和高剂量。每组6只。模型组、低剂量、中剂量和高剂量大鼠接受骨髓炎造模手术。正常组和模型组大鼠每天灌服1次蒸馏水(10ml);低剂量、中剂量、高剂量组大鼠每天灌服1次含蛇葡萄根提取物250μg/ml、500μg/ml、1000μg/ml。给药8周后处死大鼠,检测NF-kB信号通路关键基因和炎症因子表达水平,X线片评价大鼠骨髓炎疾病严重程度。【结果】RTqPCR检测,与正常组比较,模型组IL-6、IL-1β、TNF-αmRNA水显著增高(P<0.05);与模型组比较,给药的三组上述指标均显著降低(P<0.05)。与正常组比较,模型组NF-kB、IKK、PU1、FOL水平显著增高(P<0.05),IkB水平显著降低(P<0.05);与模型组相比,低剂量、中剂量和高剂量NF-kB【(1.8+0.0)vs(1.7±0.1)vs(1.4±0.1)vs(1.3±0.1),P<0.001】,IKK【(2.3±0.1)vs(2.0±0.1)vs(1.6±0.1)vs(1.3±0.0),P<0.001】、PU1【(2.6±0.1)vs(1.9±0.1)vs(1.7±0.1)vs(1.4±0.1),P<0.001】、F0LR【(2.1±0.1)vs(1.8±0.1)vs(1.5±0.1)vs(1.4±0.1),P<0.001】水平指标均显著降低,而IkB水平【(0.4±0.0)vs(0.5±0.1)vs(0.6±0.1)vs(0.8±0.0),P<0.001】显著升高,并呈剂量依赖性。Westernblot检测,与正常组比较,模型组IL-6、IL-1β、TNF-α水平显著增高(P<0.05),与模型组比较,三个给药组上述指标均显著降低(P<0.05)。【结论】蛇葡萄根提取物可以抑制NF-kB信号通路关键基因和炎症因子表达,降低炎性细胞浸润,对大鼠骨髓炎具有一定治疗作用。【Objective】To investigate the effect of radix smpelopsis sinicae extract on the healing process of chronic osteomyelitis in rat.【Methods】The rats were divided into 5 groups,including normal group,model group,low dose,medium dose and high dose,with 6 animals in each group.Of them,the rats in model group,low dose,medium dose and high dose rats underwent osteomyelitis model making operation.Normal group and model group were given distilled water(10 ml)once a day,whereas low dose,medium dose and high dose groups were giv-en 250μg/ml,500μg/ml and 1000μg/ml of radix smpelopsis sinicae extract respectively once a day.After 8 weeks of administration,the rats were killed,and the expression levels of key genes and inflammatory factors in NF-kB signaling pathway were detected,while the severi-ty of osteomyelitis was evaluated by X-ray and histology.【Results】As for RT-qPCR assay,the model group had significant increases in mRNA levels of IL-6,IL-1βand TNF-αcompared with the normal group(P<0.05);however,the three drug-given groups got significantly decreases regarding abovesaid items compared with the model group(P<0.05).In addition,the model group had significant increases in the levels of NF-kB,IKK,PU1 and FOL,while significant decline of IkB compared with the normal group(P<0.05).Compared with the model group,the low dose,middle dose and high dose groups got significant decrease in terms the nf-kappa B【(1.8±0.0)s(1.7±0.1)vs(1.4+0.1)vs(1.3±0.1),P<0.001】.IKK【(2.3±0.1)vs(2.0±0.1)vs(1.6±0.1)vs(1.3±0.0),P<0.001】,PU1【(2.6±0.1)vs(1.9±0.1)vs(1.7±0.1)vs(1.4±0.1),P<0.001】,FOLR【(2.1±0.1)vs(1.8±0.1)vs(1.5±0.1)vs(1.4±0.1),P<0.001】,whereas significant upgrading in IkB level【(0.4±0.0)vs(0.5±0.1)vs(0.6+0.1)vs(0.8±0.0),P<0.001】in dosage-dependent manner.Regarding western blot analysis,the model group had significant increase in IL-6,IL-1βand TNF-αcompared with the normal group(P<0.05),while the above indexes were significantly decreased in the three drug administration groups compare

关 键 词：蛇葡萄根 骨髓炎 NF-KB信号通路 炎症因子 

分 类 号：R681.2[医药卫生—骨科学]