An aquaporin and an aquaglyceroporin have roles in low temperature adaptation of mosquitoes (Anopheles sinensis)  

作　　者：Yong-Qiao Zhao Yi-Ying Tang Ju-Ping Hu Yu-Zheng Huang Kai Wan Mei-Hua Zhang Ju-Lin Li Guo-Ding Zhu Jian-Xia Tang 

机构地区：[1]National Health Commission Key Laboratory of Parasitic Disease Control and Prevention,Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology,Jiangsu Provincial Medical Key laboratory,Jiangsu Institute of Parasitic Diseases,Wuxi,Jiangsu,China [2]School of Public Health,Nanjing Medical University,Nanjing,Jiangsu,China [3]Wuxi School of Medicine,Jiangnan University,Wuxi,Jiangsu,China

出　　处：《Insect Science》2024年第6期1743-1755,共13页昆虫科学（英文版）

基　　金：supported by National Natural Science Foundation of China and the Bill and Melinda Gates Foundation joint program(82261128002);the National Nature Science Foundation of China(81401694);the Jiangsu Natural Science Foundation(BK2012106).

摘　　要：Mosquitoes(Anopheles sinensis),widely geographically distributed in Asia including China,are the primary vector of the malaria parasite Plasmodium vivax and other parasitic diseases such as Malayan filariasis.An.sinensis can survive through low winter temperatures.Aquaporin channels are found in all life forms,where they facilitate environmental adaptation by allowing rapid trans-cellular movement of water(classical aquaporins)or water and solutes such as glycerol(aquaglyceroporins).Here,we identified and characterized 2 aquaporin(AQP)homologs in An.sinensis:AsAQP2(An.sinensis aquaglyceroporin)and AsAQP4(An.sinensis aquaporin).When expressed in frog(Xenopus laevis)oocytes,AsAQP2 transported water,glycerol,and urea;AsAQP4 transported only water.Water permeation through AsAQP2 and AsAQP4 was inhibited by mercuric chloride.AsAQP2 expression was slightly higher in adult female mosquitoes than in males,and AsAQP4 expression was significantly higher in adult males.The 2 AsAQPs were highly expressed in Malpighian tubules and midgut.AsAQP2 and AsAQP4 expression was up-regulated by blood feeding compared with sugar feeding.At freezing point(0℃),the AsAQP4 expression level increased and An.sinensis survival time reduced compared with those at normal temperature(26℃).At low temperature(8℃),the AsAQP2 and AsAQP4 expression levels decreased and survival time was significantly longer compared with those at 26℃.These results suggest that AsAQP2 and AsAQP4 have roles in water homeostasis during blood digestion and in low temperature adaptation of A.sinensis.Together,our results show that the 2 AQPs are important for mosquito diuresis after blood feeding and when exposed to low temperatures.

关 键 词：AQUAPORIN aquaglyceroporin Anopheles sinensis blood digestion low temperature adaption 

分 类 号：Q96[生物学—昆虫学]