Using kin discrimination to construct synthetic microbial communities of Bacillus subtilis strains impacts the growth of black soldier fly larvae  

作　　者：Jun-Hui Zhao Ping Cheng Yi Wang Xun Yan Zhi-Min Xu Dong-Hai Peng Guo-Hui Yu Ming-Wei Shao 

机构地区：[1]Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs,Guangdong University Key Laboratory for Sustainable Control of Fruit and Vegetable Diseases and Pests,Innovative Institute for Plant Health,College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou,China [2]State Key Laboratory of Agricultural Microbiology,National Engineering Research Center of Microbial Pesticides,College of Life Science and Technology,Huazhong Agricultural University,Wuhan,China [3]Hubei Hongshan Laboratory,Wuhan,China [4]College of Resources and Environment,Zhongkai University of Agriculture and Engineering,Guangzhou,China

出　　处：《Insect Science》2024年第6期1943-1959,共17页昆虫科学（英文版）

基　　金：supported by the Key-Area Research and Development Program of Guangdong Province(2020B0202080002).

摘　　要：Using synthetic microbial communities to promote host growth is an effective approach.However,the construction of such communities lacks theoretical guidance.Kin discrimination is an effective means by which strains can recognize themselves from non-self,and construct competitive microbial communities to produce more secondary metabolites.However,the construction of cooperative communities benefits from the widespread use of beneficial microorganisms.We used kin discrimination to construct synthetic communities(SCs)comprising 13 Bacillus subtilis strains from the surface and gut of black soldier fly(BSF)larvae.We assessed larval growth promotion in a pigeon manure system and found that the synthetic community comprising 4 strains(SC 4)had the most profound effect.Genomic analyses of these 4 strains revealed that their complementary functional genes underpinned the robust functionality of the cooperative synthetic community,highlighting the importance of strain diversity.After analyzing the bacterial composition of BSF larvae and the pigeon manure substrate,we observed that SC 4 altered the bacterial abundance in both the larval gut and pigeon manure.This also influenced microbial metabolic functions and co-occurrence network complexity.Kin discrimination facilitates the rapid construction of synthetic communities.The positive effects of SC 4 on larval weight gain resulted from the functional redundancy and complementarity among the strains.Furthermore,SC 4 may enhance larval growth by inducing shifts in the bacterial composition of the larval gut and pigeon manure.This elucidated how the SC promoted larval growth by regulating bacterial composition and provided theoretical guidance for the construction of SCs.

关 键 词：community construction genomic analysis gut bacteria pigeon manure 

分 类 号：Q93[生物学—微生物学] Q96