施磷和接种丛枝菌根真菌对钒胁迫下玉米生长的影响  

Effects of arbuscular mycorrhizal fungi inoculation and phosphorus application on maize growth under vanadium stress

作　　者：裘浪 王志刚[3] 俞龙生[4] 张云云 余浩 张延旭[5] QIU Lang;WANG Zhigang;YU Longsheng;ZHANG Yunyun;YU Hao;ZHANG Yanxu(School of Environment and Surveying Engineering,Suzhou University,Suzhou 234000,Anhui,China;Institute of Eco-environmental and Soil Sciences,Guangdong Academy of Sciences,Guangzhou 510650,China;Cllege of Geoscience and Surveying Enginering,China University of Mining and Technology-Bejing,Beijing100083,China;Guangzhou Research Institute of Environmental Protection,Guangzhou 510620,China;State Key Laboratory for Fine Exploration and Intelligent Development of Coal Resources,China University of Mining and Technology-Beijing,Beijing 100083,China)

机构地区：[1]宿州学院环境与测绘工程学院,安徽宿州234000 [2]广东省科学院生态环境与土壤研究所,广州510650 [3]中国矿业大学(北京)地球科学与测绘工程学院,北京100083 [4]广州市环境保护科学研究院有限公司,广州510620 [5]中国矿业大学(北京)煤炭精细勘探与智能开发全国重点实验室,北京100083

出　　处：《应用生态学报》2024年第12期3444-3452,共9页Chinese Journal of Applied Ecology

基　　金：安徽省自然科学基金项目(2308085QD117);安徽省教育厅高校科学研究项目(2022AH051385);宿州学院博士科研启动基金项目(2023BSK060)资助。

摘　　要：钒(V)与磷(P)具有类似的化学结构,而丛枝菌根真菌(AMF)对P元素具有高度的亲和性。为了解钒胁迫下接种AMF和施磷对玉米生长的影响,采用玉米盆栽试验,在钒处理(0、100、200和400mg·kg^(-1))下接种AMF和施P(0、20和50mg·kg^(-1)),测定玉米生长、P与V吸收以及植株体内钒形态分布。结果表明:与对照相比,钒胁迫(100、200和400mg·kg^(-1))显著降低了玉米根系AMF侵染率和生物量,其中200和400mg·kg^(-1)钒水平下的AMF侵染率分别显著降低14.9%~28.0%和29.5%~31.9%,生物量相应降低41.4%~42.2%和54.3%~55.3%;钒胁迫显著抑制玉米地上部对P的吸收,提高了地上部和根系V浓度和吸收量。接种AMF和施磷能够缓解钒胁迫对玉米生长的抑制作用,显著降低玉米V富集系数(23.3%~47.7%)和转运系数(13.1%~27.9%)以及玉米V吸收量,提高玉米地上部和根系生物量以及P吸收量。接种AMF与施磷对玉米根系AMF侵染率、地上部的生物量和P、V吸收量具有显著交互作用。分析玉米植株体内金属V形态发现,接种AMF可促进玉米将吸收的高毒性V还原为低毒性V。接种AMF主要通过促进玉米生长和P吸收、抑制V吸收来缓解钒胁迫,施磷则可进一步起到改善作用。t Vanadium(V)and phosphorus(P)are similar in chemical structure.Arbuscular mycorrhizal fungi(AMF)have high affinity for P.To investigate the effects of AMF inoculation and P application on maize growth under V stress,we investigated the effects of AMF inoculation and P application(O,20 and 50 mg·kg^(-1)P,O,)on maize growth,P and V uptake,and V speciation in the soil amended with four levels of V addition(O,100,200,and 400 mg·kg^(-1)V).The results showed that V stress(100,200 and 400 mg·kg^(-1))significantly de-creased root mycorrhizal colonization and plant biomass.The 200 and 400 mg·kg^(-1)V stress treatments significantly decreased root mycorrhizal colonization by 14.9%-28.0%and 29.5%-31.9%,and reduced plant biomass by 41.4%-42.2%and 54.3%-55.3%,respectively.V stress significantly decreased P concentration and uptake of maize shoot and increased V concentration and uptake of maize shoot and root.AMF inoculation and P application could alleviate the inhibition effect of V on maize growth,which significantly decreased bioconcentration factor of V by 23.3%-47.7%and translocation factor of V by 13.1%-27.9%,as well as V concentration and uptake of maize shoot,but improved plant biomass and P uptake of maize shoot and root.The factor of AMF inoculation and Pap-plication had interaction on root mycorrhizal colonization and plant biomass,as well as P and V uptake of maize shoot.AMF inoculation promoted the reduction of Vy into Viv in plant tissues.AMF inoculation could alleviate V stress by promoting maize growth and P uptake and inhibiting V translocation,with synergistic effects from P application.

关 键 词：钒 丛枝菌根真菌 磷吸收 侵染率 迁移转运 

分 类 号：S513[农业科学—作物学]