芪桂复元汤通过减轻神经炎症减少大鼠脑缺血再灌注损伤  

Qigui Fuyuan Decoction alleviating cerebral ischemia-reperfusion injury by attenuating neuroinflammation

作　　者：周玉英 翁国虎 顾勇 Zhou Yuying;Weng Guohu;Gu Yong(Department of Clinical Research Center,Affiliated Chinese Medicine Hospital of Hainan Medical University,Haikou 570203,China;Department of Clinical Research Center,Hainan Hospital,Guangdong Provincial Hospital of Chinese Medicine,Haikou 570203,China;Department of Encephalopathy,Hainan Hospital,Guangdong Provincial Hospital of Chinese Medicine,Haikou 570203,China)

机构地区：[1]海南医科大学附属中医院临床研究中心,海口570203 [2]广东省中医院海南医院临床研究中心,海口570203 [3]广东省中医院海南医院脑病科,海口570203

出　　处：《国际中医中药杂志》2024年第12期1601-1607,共7页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(82160247、82360255、82260923);海南省临床研究中心项目(LCYX202104);海南省重大科技计划项目(ZDKJ2021034)。

摘　　要：目的探讨芪桂复元汤改善脑缺血再灌注损伤大鼠的神经功能、减轻神经损伤和炎症反应的作用机制。方法以线栓法建立大鼠大脑中动脉梗塞(MCAO)模型,按随机数字表法分为假手术组、模型组及芪桂复元汤低、中、高剂量组。采用改良大鼠神经缺损评分评价神经功能缺损,TTC染色评估脑梗死体积,Western blot法检测TNF-α、IL-6、IL-1β、诱导型一氧化氮合酶(iNOS)蛋白表达,ELISA法检测血清TNF-α、IL-6水平。以L-谷氨酸刺激PC12细胞建立体外神经损伤模型,制备芪桂复元汤含药血清和含药肠吸收液,利用细胞计数试剂盒-8(CCK-8)检测其对细胞损伤的影响。结果与模型组比较,芪桂复元汤低、中、高剂量组大鼠神经功能缺损症状评分降低(P<0.01),脑梗死体积减少(P<0.01),血清IL-6、TNF-α水平降低(P<0.05),脑组织IL-1β、IL-6、iNOS、TNF-α蛋白表达降低(P<0.05);芪桂复元汤含药血清和肠吸收液均可减轻L-Glu诱导的PC12细胞损伤(P<0.01)。在体外实验中,30 mmol/L的L-谷氨酸干预24 h可引起PC12细胞损伤,芪桂复元汤含药血清和肠吸收液均可减轻L-Glu诱导的PC12细胞损伤(P<0.01)。结论芪桂复元汤可减轻MCAO大鼠脑缺血再灌注损伤、改善神经功能并抑制神经炎症。Objective To explore the effects of Qigui Fuyuan Decoction(QGFYD)on improving neural function,reducing neuronal damage and inflammatory response in rats with cerebral ischemia-reperfusion injury.Methods A middle cerebral artery occlusion(MCAO)model was established in SD rats using suture occlusion method.The rats were divided into sham-operation group,model group,and QGFYD low-(QGFYD-L),medium-(QGFYD-M),and high-dosage(QGFYD-H)groups according to random number table method.The modified neurological severity scores(mNSS)was used to evaluate the neurological deficit score,Triphenyl tetrazolium chloride(TTC)staining to assess the volume of cerebral infarction,and Western blot for inflammatory factor expression and ELISA assay for serum inflammatory factor levels.Stimulation of PC12 cells with L-glutamic acid(L-Glu)was used to establish an in vitro neurological injury model,and QGFYD-containing serum and intestinal absorptive solution were prepared to study its neuroprotective effects in vitro using the CCK-8 assay kit.Results Compared with the model group,QGFYD-L,QGFYD-M,and QGFYD-H groups showed a decrease in neurological deficit symptom scores(P<0.01),a decrease in cerebral infarction volume(P<0.01),a decrease in serum IL-6 and TNF-αlevels(P<0.05),and a decrease in brain tissue IL-1β,IL-6,iNOS,and TNF-αprotein expression(P<0.05);both the QGFYD-containing serum and the intestinal absorption solution could alleviate L-Glu induced damage to PC12 cells(P<0.01).In vitro experiments showed that intervention with 30 mmol/L L-glutamate for 24 hours could cause damage to PC12 cells.Both the serum containing Qigui Fuyuan decoction and intestinal absorption solution could alleviate L-Glu-induced damage to PC12 cells(P<0.01).Conclusion Qigui Fuyuan Decoction can alleviate cerebral ischemia-reperfusion injury,improve neurological function,and inhibit neuroinflammation.

关 键 词：脑缺血 再灌注损伤 神经炎症 芪桂复元汤 大鼠 

分 类 号：R285.5[医药卫生—中药学]