一测多评法对沼生忍冬叶质量分析研究  

Study on quality analysis of Lonicera alberti Regel.Leaves by QAMS

作　　者：陈燕 韩会靖 姚雨含 赵军 徐芳 李晨阳 Chen Yan;Han Huijing;Yao Yuhan;Zhao Jun;Xu Fang;Li Chenyang(Plant Chemistry Research Laboratory,Key Lab.of Xinjiang Uygur Medicine,Xinjiang Institute of Materia Medica,Urumqi 830011,China;Division of Businesses Management,Xinjiang Uygur Autonomous Region Institute for Drug Control,Urumqi 830054,China)

机构地区：[1]新疆维吾尔自治区药物研究所植物化学研究室新疆维吾尔自治区维吾尔药重点实验室,乌鲁木齐830011 [2]新疆维吾尔自治区药品检验研究院业务管理部,乌鲁木齐830054

出　　处：《国际中医中药杂志》2024年第12期1629-1635,共7页International Journal of Traditional Chinese Medicine

基　　金：新疆维吾尔自治区卫生健康青年医学科技人才专项科研项目(WJWY202128)。

摘　　要：目的建立一测多评法(QAMS)测定沼生忍冬叶中绿原酸、新绿原酸、隐绿原酸、异绿原酸A、异绿原酸C、芦丁、莫诺苷及木犀草苷8个成分含量,并验证该方法在沼生忍冬叶质量评价中应用的可行性与适用性。方法采用Agilent Eclipse XDB-C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1%甲酸水溶液为流动相,梯度洗脱,检测波长258 nm,体积流量1.0 ml/min,柱温30℃,进样量为10μl。结果绿原酸、新绿原酸、隐绿原酸、异绿原酸A、异绿原酸C、芦丁、莫诺苷及木犀草苷8个成分在相应浓度范围内线性关系良好(r≥0.9996),平均加样回收率分别为103.16%、103.98%、99.49%、103.78%、102.74%、101.12%、104.62%、100.94%,RSD分别为1.30%、1.63%、2.92%、2.10%、1.27%、2.40%、1.15%、2.76%。以绿原酸为内参成分,分别计算异绿原酸A、异绿原酸C、新绿原酸、隐绿原酸、莫诺苷、木犀草苷、芦丁相对校正因子分别为0.7855、0.6939、1.0015、1.0872、1.2339、0.3691、0.5075。QAMS法和外标法的含量测定结果表明,除莫诺苷外,其他6个成分比较差异无统计学意义。结论所建立的HPLC含量测定方法可用于沼生忍冬叶药材的质量控制;一测多评法可用于测定沼生忍冬叶中新绿原酸、隐绿原酸、异绿原酸A、异绿原酸C、芦丁及木犀草苷含量。Objective To establish the QAMS method for content determination of eight chemical compositions(chlorogenic acid,neochlorogenic acid,cryptochlorogenin acid,isochlorogenic acid A,isochlorogenic acid C,rutin,morroniside and luteolin-7-O-glucoside)from Lonicera alberti Regel.leaves;To verify the feasibility and applicability of this method in quality control for Lonicera alberti Regel.leaves.Methods The HPLC analysis was performed on a Agilent Eclipse XDB-C18(250 mm×4.6 mm,5μm)with a mobile phase consisted of acetonitrile and 0.1%formic acid solution in gradient elution manner at a flow rate of 1 ml/min.The column temperature was maintained at 30℃and the detection wavelength was set at 258 nm.The injection volume was 10μl.Results Chlorogenic acid,neochlorogenic acid,cryptochlorogenin acid,isochlorogenic acid A,isochlorogenic acid C,rutin,morroniside and luteolin-7-O-glucoside had a good linear relationship in the corresponding concentration range(r≥0.9996).The average sample recovery rates were 103.16%,103.98%,99.49%,103.78%,102.74%,101.12%,104.62%,and 100.94%,respectively.The RSD values were 1.30%,1.63%,2.92%,2.10%,1.27%,2.40%,1.15%,and 2.76%,respectively.Chlorogenic acid was set as internal reference substance,the relative correction factors of isochlorogenic acid A,isochlorogenic acid C,neochlorogenic acid,cryptochlorogenin acid,morroniside,luteolin-7-O-glucoside,rutin were 0.7855,0.6939,1.0015,1.0872,1.2339,0.3691,0.5075,respectively.The content determination results of QAMS method and external standard method showed that there was no statistical significance in the comparison of the other six components except for morroniside.Conclusions The established HPLC method can be used for the quality control of Lonicera alberti Regel.leaves.QAMS can be used to determine the contents of neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A,isochlorogenic acid C,rutin and luteolin-7-O-glucoside in Lonicera alberti Regel.leaves.

关 键 词：忍冬叶 一测多评法 相对校正因子 含量测定(中药) 质量控制 

分 类 号：R284.1[医药卫生—中药学]