Advances in virus-host interaction research based on microfluidic platforms  

作　　者：Cheng Wang Ji Wang Dong Liu Zhi-Ling Zhang 

机构地区：[1]School of Life Sciences,Co-Innovation Center of Neuroregeneration,Nantong Laboratory of Development and Diseases,Nantong University,Nantong 226019,China [2]College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072,China [3]Centre for Immunology and Infection,Hong Kong Science and Technology Park,Hong Kong SAR 999077,China

出　　处：《Chinese Chemical Letters》2024年第12期125-137,共13页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.22274118,22074107,and 81870359);the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(No.22KJB320020);InnoHK funding(C2i)from Innovation&Technology Commission(ITC)of Hong Kong SAR Government。

摘　　要：Viral epidemics pose a serious threat to global public health,making it essential to explore virus-host interactions for uncovering the pathogenesis of viral diseases and developing effective antiviral strategies.Traditional in vitro cell infection models struggle to replicate physiological microenvironment,while animal infection models may encounter obstacles such as species gap,high-cost,and ethical issues.Additionally,potential heterogeneous infection outcomes are usually inaccessible by population-based experiments.Microfluidics,as an emerging interdisciplinary platform,has proven to be a powerful tool for inquiring virus-host interactions.In this review,conventional virological methods were introduced first and remarkable advantages of microfluidics in viral cell biology were highlighted.Next,the in-depth applications of microfluidics in analyzing heterogeneity of virus-host interplays,dynamic monitoring of events related to viral life cycle,and modeling of viral infectious diseases were fully elaborated from the perspective of single-cell chip,multi-cell culture chip and organ-on-a-chip(organ chip).Finally,the opportunities and challenges in developing robust microfluidic methods for virology were discussed.Overall,this review aims to provide an overview of microfluidic-based research on virus-host interaction and promote multidisciplinary collaborations for better understanding and responding to viral threats.

关 键 词：MICROFLUIDICS Virus-host interaction Cellular microchip Single-cell analysis Organ-on-a-chip Viral infectious disease 

分 类 号：R373[医药卫生—病原生物学]