肠上皮细胞分离纯化及鉴定的研究进展  

Advances in the Isolation and Purification of Intestinal Epithelial Cells and Their Identification

作　　者：曹佳龙 蔺雅楠 云斯琴 方心兰 杜明 苏少锋 刘源壹 王娜 白东义[1] 赵一萍[1] CAO Jialong;LIN Yanan;YUN Siqin;FANG Xinlan;DU Ming;SU Shaofeng;LIU Yuanyi;WANG Na;BAI Dongyi;ZHAO Yiping(Key Laboratory of Equus Germplasm Innovation,Ministry of Agriculture and Rural Affairs,Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation,Equus Research Center,Inner Mongolia Agricultural University,College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China;Inner Mongolia Academy of Agricultural and Animal Husbandry Sceinces,Hohhot 010031,China)

机构地区：[1]农业农村部马属动物种业创新重点实验室/内蒙古自治区马属动物科学研究与技术创新重点实验室/内蒙古农业大学马属动物研究中心/内蒙古农业大学动物科学学院,呼和浩特010018 [2]内蒙古自治区农牧业科学院,呼和浩特010031

出　　处：《内蒙古农业大学学报（自然科学版）》2024年第6期94-100,共7页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金项目(31860636);内蒙古自治区重点研发项目(2023YFDZ0002)。

摘　　要：肠上皮细胞(IEC)是肠道黏膜屏障的重要组成部分,它可以通过分泌各种抗菌抗病毒的物质及调节免疫反应来防御肠道炎症反应。基于IEC在调节炎症反应中的重要作用,分离和培养高质量、高纯度的IEC可以作为研究肠道炎症相关机制的重要细胞模型。随着技术的不断发展和成熟,对IEC的研究应用范围也在不断扩大。因此,本文总结了IEC的主要功能及研究中常用的分离、纯化、鉴定方法,以期为利用动物IEC开展肠道疾病领域的相关研究提供参考。Intestinal epithelial cells(IEC)are crucial components of the intestinal mucosal barrier,which can defend against intesti⁃nal inflammatory responses by secreting various antibacterial and antiviral substances and regulating immune responses.Based on the important role of IEC in regulating the inflammatory response,the isolation and culture of high-quality and high-purity IEC can serve as an important cell model for studying the mechanisms related to intestinal inflammation.With the continuous development and maturation of technology,the range of research applications for IEC continues to expand.Therefore,this paper summarized the main functions of IEC and the commonly used isolation,purification and identification methods in research,aiming to provide a ref⁃erence for utilizing animal IEC in related research within the field of intestinal diseases.

关 键 词：肠上皮细胞 分离 纯化 鉴定 

分 类 号：S813[农业科学—畜牧学]