四川省三带喙库蚊流行性乙型脑炎病毒携带情况调查  

Investigation on Japanese encephalitis virus carriedby Culex tritaeniorhynchus in Sichuan Province

作　　者：张镇川 李宇斯 余技钢 杜森焱 朱茜 李观翠 李玲玲 胡雅劼[1] ZHANG Zhenchuan;LI Yusi;YU Jigang;DU Senyan;ZHU Xi;LI Guancui;LI Lingling;HU Yajie(Institute for Prevention and Control of Parasitic Diseases,Sichuan Center for Disease Control and Prevention,Chengdu,Sichuan 610041,China;College of Veterinary Medicine,Sichuan Agricultural University,Chengdu,Sichuan 611130,China)

机构地区：[1]四川省疾病预防控制中心寄生虫病预防控制所,四川成都610041 [2]四川农业大学动物医学院,四川成都611130

出　　处：《中华卫生杀虫药械》2024年第6期564-567,共4页Chinese Journal of Hygienic Insecticides and Equipments

基　　金：四川省疾病预防控制中心科研基金项目(ZX202411)。

摘　　要：目的调查四川省三带喙库蚊乙脑病毒携带情况,研究乙脑病毒分布的基本特征,为预测乙脑流行风险及科学防控提供依据。方法2023年5—8月在四川省农村地区采集三带喙库蚊标本,将单只雌蚊研磨后提取RNA,反转录为cDNA,使用基于TaqMan探针的荧光定量RT-PCR方法检测蚊虫标本中的乙脑病毒,采用卡方检验进行蚊虫感染率的比较,两两比较采用Bonferrori法。结果2023年在四川省共采集蚊虫标本1070只,其中178只蚊虫标本检测结果为乙脑病毒阳性,总体感染率为16.64%。以采样地区来看,川南和川东部分城市感染率较高;以采样生境来看,各生境感染率差异显著(χ^(2)=27.012,P<0.05),猪圈采样蚊虫感染率(13.47%)显著低于牛圈(21.85%)和农户(39.53%);以采样月份来看,5月采样蚊虫感染率(30.56%)和8月采样蚊虫感染率(26.21%)显著高于6月(13.03%)和7月(6.68%)。结论四川省三带喙库蚊乙脑病毒感染率较高,农村地区是乙脑病毒重要的自然疫源地。Objective To investigate the carrying situation of Japanese encephalitis virus of Culex tritaeniorhynchus in Sichuan Province,and to study the basic characteristics of the distribution of Japanese encephalitis virus,so as to provide a basis for predicting the epidemic risk of Japanese encephalitis and scientific prevention and control.Methods Samples of Culex tritaeniorhynchus were collected from rural areas of Sichuan Province from May to August 2023.RNA was extracted from a single female mosquito and reverse transcribed into cDNA.Japanese encephalitis virus in mosquito samples was detected by fluorescence quantitative RT-PCR based on TaqMan probe.The Chi-square test was used to compare the mosquito infection rate,and the Bonferroni test was used for pairwise comparison.Results A total of 1070 mosquito samples were collected in 2023,of which 178 mosquito samples were positive for Japanese encephalitis virus,with an overall infection rate of 16.64%.According to the sampling area,the infection rate was higher in some cities in southern and eastern Sichuan.According to the sampling habitat,the infection rate was significantly different among different habitats(χ^(2)=27.012,P<0.05).The mosquito infection rate in pigsty(13.47%)was significantly lower than that in cattle pens(21.85%)and farmers(39.53%).According to the sampling month,the mosquito infection rate in May(30.56%)and August(26.21%)were significantly higher than that in June(13.03%)and July(6.68%).Conclusion The infection rate of Japanese encephalitis virus of Culex tritaeniorhynchus is high in Sichuan Province,and the rural area is an important natural focus of Japanese encephalitis virus.

关 键 词：三带喙库蚊 流行性乙型脑炎病毒 感染率 

分 类 号：R184.31[医药卫生—流行病学] R183.5[医药卫生—公共卫生与预防医学]