蜗牛酶对酸枣叶醇提物生物活性的影响  

Effect of Snailase on the Bioactivity of Ethanol Extracts from Ziziphus jujuba var.spinosa Leaves

作　　者：张菊 周春伶 余梦媛 张冉冉 田溪 贾东升[2] ZHANG Ju;ZHOU Chunling;YU Mengyuan;ZHANG Ranran;TIAN Xi;JIA Dongsheng(College of Bioscience and Engineering,Hebei University of Economics and Business,Shijiazhuang 050061,Hebei,China;Institute of Cash Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050041,Hebei,China)

机构地区：[1]河北经贸大学生物科学与工程学院,河北石家庄050061 [2]河北省农林科学院经济作物研究所,河北石家庄050041

出　　处：《武汉大学学报（理学版）》2024年第5期638-648,共11页Journal of Wuhan University:Natural Science Edition

基　　金：河北省自然科学基金青年科学基金(H2020207002);河北省中央引导地方科技发展资金(226Z2801G);河北省人社厅择优资助博士后科研重点项目(B2022005014);河北经贸大学科研基金(2021SGYB04);河北省农林科学院科技创新项目(C21R0803,C22R0802)。

摘　　要：采用蜗牛酶对酸枣叶醇提物进行酶解,比较了酶解前后样品的体内外活性差异,并结合超高效液相色谱-质谱联用技术(UPLC-MS)进行成分分析,以揭示蜗牛酶对酸枣叶醇提物生物活性的影响。结果显示,酶解后的酸枣叶醇提物在体内外均具有更好的抗氧化活性,且神经保护作用显著。蜗牛酶主要影响了酸枣叶中黄酮、有机酸、苯丙素、糖类等化合物的含量;酸枣叶醇提物的生物活性与黄酮苷元、二羟基苯甲酸、简单苯丙素、糖酸等化合物的含量呈正相关。据此推测,蜗牛酶通过断裂酸枣叶中化合物的糖苷键增加有效活性成分含量,从而提高其生物活性。The enzymatic hydrolysis of the ethanol extracts from Ziziphus jujuba var.spinosa leaves was carried out by snailase.In order to reveal the effect of snailase on the biological activity of the extracts,the differences of in vitro and in vivo activities of the samples before and after enzymatic hydrolysis were compared.And the composition changes were analyzed by ultra performance liquid chromatography-mass spectrometry(UPLC-MS).The results showed that the enzymatic hydrolyzed ethanol extracts of Ziziphus jujuba var.spinosa leaves had better antioxidant activity both in vitro and in vivo,and had significant neuroprotective effect.UPLC-MS analysis and Spearman correlation analysis showed that enzymatic hydrolysis mainly affected the content of flavonoids,organic acids,phenylpropanes,carbohydrates and other compounds.The biological activity was positively correlated with the content of flavonoid aglycones,dihydroxybenzoic acid,simple phenylpropanoids and saccharic acid.It is speculated that snailase can increase the content of active ingredients by breaking the glucoside bonds of compounds in Ziziphus jujuba var.spinosa leaves,so as to improve the biological activity.

关 键 词：酸枣叶 酶解 抗氧化活性 神经保护 

分 类 号：R96[医药卫生—药理学]