桔梗抑制支原体肺炎上皮间质转化的机制研究  

Mechanism of Platycodonis radix in Inhibiting Epithelial-Mesenchymal Transition in Mycoplasma Pneumoniae

作　　者：胡鹏 蒙艳丽 唐静 王晓溪 王欣 马鸿菲 王伟明 HU Peng;MENG Yanli;TANG Jing;WANG Xiaoxi;WANG Xin;MA Hongfei;WANG Weiming(Heilongjiang Academy of Traditional Chinese Medicine,Harbin 150036,China)

机构地区：[1]黑龙江省中医药科学院,黑龙江哈尔滨150036

出　　处：《中医药学报》2025年第1期7-13,共7页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金面上项目(82174060);黑龙江省自然科学基金项目(LH2021H074);黑龙江省卫生健康委科研课题(20210202040011);黑龙江中医药科研项目(ZHY2022-075)。

摘　　要：目的:探究桔梗对肺炎支原体感染Beas-2B细胞和小鼠的治疗作用及机制。方法:Beas-2B细胞分组,建立支原体肺炎模型,给药1 d。细胞HE染色观察各组细胞生长状态;MTT和CCK-8法检测细胞存活情况。60只BALB/c小鼠随机分为空白组、模型组、阿奇霉素组、桔梗高剂量组、桔梗中剂量组、桔梗低剂量组,每组10只。滴鼻法建立小鼠支原体肺炎模型,HE染色观察肺部病理情况,免疫组织化学法和RT-PCR法分别检测AQP1、Wnt-5α、β-catenin的蛋白和mRNA表达水平;ELISA法检测血清SOD、TNF-α、IL-6水平;RT-PCR检测AQP1抑制剂对Beas-2B细胞AQP1、Wnt-5α、β-catenin的mRNA表达量的影响。结果:模型组Beas-2B细胞失去原有细胞形态,数量减少;小鼠肺组织AQP1蛋白和mRNA表达量降低(P<0.05,P<0.01),Wnt-5α、β-catenin蛋白和mRNA表达量升高(P<0.05,P<0.01);血清SOD表达量下降(P<0.01),血清TNF-α、IL-6表达量升高(P<0.01)。与模型组相比,桔梗显著改善Beas-2B细胞的生长状态;小鼠肺组织AQP1蛋白和mRNA表达量升高(P<0.05,P<0.01),Wnt-5α、β-catenin蛋白和mRNA表达量降低(P<0.05,P<0.01),血清SOD均升高而TNF-α、IL-6表达量均下降(P<0.05,P<0.01);与空白组相比,AQP1抑制剂抑制Beas-2B细胞AQP1的mRNA表达量(P<0.01),上调Wnt-5α、β-catenin的mRNA表达量(P<0.05)。结论:桔梗基于Wnt/β-catenin信号通路调控AQP1的表达对肺炎支原体引起的肺组织上皮间质转化发挥抑制作用,进而保护肺组织。Objective:To investigate the therapeutic effect of Platycodonis radix on mycoplasma pneumoniae infection in Beas-2B cells and mice.Methods:Beas-2B cells were divided into groups,and a mycoplasma pneumonia model was established,with drug administration for 1 day.HE staining was used to observe the cell growth status in each group,and MTT and CCK-8 assays were used to assess cell viability.Sixty BALB/c mice were randomly divided into six groups:blank group,model group,azithromycin group,high-dose,medium-dose,and low-dose Platycodonis radix groups,with 10 mice per group.A Mycoplasma pneumonia model was established in mice using the nasal drop method.Lung pathology was observed using HE staining,and the expression levels of AQP1,Wnt-5α,andβ-catenin were detected by immunohistochemistry and RT-PCR.Elisa was used to measure serum SOD,TNF-α,and IL-6 levels.RT-PCR was used to detect the effect of AQP1 inhibitor on the mRNA expression of AQP1,Wnt-5α,andβ-catenin in Beas-2B cells.Results:In the model group,Beas-2B cells lost their original morphology and decreased in number.The protein and mRNA expression levels of AQP1 in mouse lung tissues were reduced(P<0.05,P<0.01),while the protein and mRNA levels of Wnt-5αandβ-catenin were increased(P<0.05,P<0.01).Serum SOD levels decreased,and serum TNF-αand IL-6 levels increased(P<0.05,P<0.01).Compared with the model group,Platycodonis radix significantly improved the growth state of Beas-2B cells.The expression of AQP1 protein and mRNA in mouse lung tissues increased(P<0.05,P<0.01),while Wnt-5αandβ-catenin protein and mRNA levels decreased(P<0.05,P<0.01).Serum SOD levels increased,and TNF-αlevels decreased(P<0.05,P<0.01).Compared with the blank group,AQP1 inhibitors suppressed AQP1 mRNA expression in Beas-2B cells(P<0.05,P<0.01),leading to the upregulation of Wnt-5αandβ-catenin mRNA expression(P<0.05,P<0.01).Conclusion:Platycodonis radix inhibits epithelial-mesenchymal transition in lung tissues caused by Mycoplasma pneumoniae by regulating AQP1 expression through the

关 键 词：桔梗 AQP1 WNT/Β-CATENIN信号通路 肺炎支原体肺炎 BEAS-2B细胞 

分 类 号：R285.5[医药卫生—中药学]