酶法催化高酸值米糠油脱臭馏出物甲酯化  

Enzymatic catalysis for methyl esterification of high acid value rice bran oil deodorized distillate

作　　者：周游[1] 李攀登[1] 贺雷雨[1] 孙丽慧[1] ZHOU You;LI Pandeng;HE Leiyu;SUN Lihui(School of Chemical Engineering,Ocean and Life Sciences,Dalian University of Technology,Panjin 124221,Liaoning,China)

机构地区：[1]大连理工大学化工海洋与生命学院,辽宁盘锦124221

出　　处：《山东大学学报（理学版）》2024年第11期85-92,共8页Journal of Shandong University(Natural Science)

基　　金：辽宁省自然科学基金项目(2019-MS-044)。

摘　　要：为了提高米糠油脱臭馏出物的利用率,本研究采用脂肪酶Eversa Transform 2.0催化米糠油脱臭馏出物甲酯化,以酯化率和体系中游离脂肪酸的含量为指标,通过单因素实验研究反应时间、加酶量、醇油摩尔比和含水量的影响,在此基础上采用响应面实验对甲酯化条件进行优化。结果表明米糠油脱臭馏出物甲酯化的最佳反应条件:酶添加量为1.2%;醇油摩尔比为5.9∶1;含水量为11.8%;反应时间为9.6 h。在此条件下酯化率为97.37%。综上,廉价的液体脂肪酶Eversa Transform 2.0催化米糠油脱臭馏出物甲酯化,可以得到较高的酯化率,为体系中植物甾醇、维生素E等活性成分的分离提取提供了绿色高效的预处理方法。In order to improve the utilization rate of rice bran oil deodorized distillate,methyl esterification of rice bran oil deodorized distillate was catalyzed by the liquid lipase Eversa Transform 2.0.The esterification rate and the content of free fatty acids in the system were used as the evaluation indicators.The effects of reaction time,enzyme amount,molar ratio of alcohol to oil,and water content on the esterification rate were studied by single factor experiments.On this basis,the response surface experiment was used to optimize the esterification conditions.The results showed that the optimal conditions for methyl esterification of rice bran oil deodorized distillate were 1.2%enzyme addition,5.9∶1 molar ratio of alcohol to oil,11.8%water content,and 9.6 h reaction time.Under these conditions,the esterification rate was 97.37%.In summary,the low-cost liquid lipase Eversa Transform 2.0 catalyzed the esterification of rice bran oil deodorized distillate,which could obtain a high esterification rate and provide a green and efficient pretreatment method for the separation and extraction of phytosterols,vitamin E,and other active components in the system.

关 键 词：米糠油脱臭馏出物 高酸值油 酶催化 响应面法 甲酯化 

分 类 号：Q503[生物学—生物化学]