211份四川小麦品种(系)矮秆基因分子检测  

Molecular Detection of Dwarf Genes in 211 Sichuan Wheat Varieties(Lines)

作　　者：丁传光 张华 陈浩[1] 何员江 顾莎莎 李晴 周新力[1] 袁国强 任勇 DING Chuanguang;ZHANG Hua;CHEN Hao;HE Yuanjiang;GU Shasha;LI Qing;ZHOU Xinli;YUAN Guoqiang;REN Yong(Wheat Research Institute/School of Life Sciences and Engineering,Southwest University of Science and Technology,Mianyang 621010,Sichuan,China;Mianyang Academy of Agricultural Science/Crop Characteristic Resources Creation and Utilization Key Laboratory of Sichuan Province,Mianyang 621023,Sichuan,China)

机构地区：[1]西南科技大学生命科学与工程学院/小麦研究所,四川绵阳621010 [2]绵阳市农业科学研究院/厅市共建作物特色资源创制及应用四川省重点实验室,四川绵阳621023

出　　处：《四川农业大学学报》2024年第6期1232-1240,I0001-I0004,共13页Journal of Sichuan Agricultural University

基　　金：国家小麦产业技术体系绵阳综合试验站(CARS-03-82);四川省育种攻关项目(2021YFYZ0002)。

摘　　要：【目的】了解四川地区小麦品种(系)的矮秆基因分布情况,以及不同矮秆基因聚合对小麦株高、穗长和节间长度的影响。【方法】以211份小麦品种(系)为材料,于2022—2023年和2023—2024年在田间调查株高、穗长和节间长度,并利用分子标记检测了矮秆基因Rht-B1b、Rht-D1b、Rht5、Rht8、Rht9、Rht10、Rht12和Rht13。【结果】在211份材料中携带Rht-B1b、Rht-D1b、Rht5、Rht8、Rht9、Rht12和Rht13的材料分布频率分别为81.0%、95.7%、28.4%、51.2%、29.4%、19.9%和8.1%,所有材料均未检测到Rht10,共得到43个不同基因的组合。矮秆基因降秆效应为Rht9>Rht5>Rht12>Rht8>Rht13,推广品种和后备品系的株高矮化效应为9.8%和9.3%,两者均为第4节(基部第2节)节间矮化效应值最大,分别为22.27%和25.91%。【结论】四川地区小麦中矮秆基因应用最广泛的是Rht-B1b、Rht-D1b和Rht8,另外,Rht5、Rht9和Rht12的应用正在提高。【Objective】This study investigates the distribution of dwarf genes in wheat varieties(lines)in Sichuan,and assesses the effects of dwarf genes aggregation on plant height,spike length and inter‐node length in wheat.【Method】The plant height,spike length and internode length of 211 wheat variet‐ies(lines)were measured during the 2022-2023 and 2023-2024 growing seasons.Dwarf genes Rht B1b,Rht-D1b,Rht5,Rht8,Rht9,Rht10,Rht12,and Rht13 were identified using molecular markers.【Result】The distribution frequencies of Rht-B1b,Rht-D1b,Rht5,Rht8,Rht9,Rht12,and Rht13 among the 211 materials were 81.0%,95.7%,28.4%,51.2%,29.4%,19.9%,and 8.1%,respectively.Rht10 was not detected in any of the materials.A total of 43 different gene combinations were identified.The ranking of culm reduction effects of the dwarf genes was Rht9>Rht5>Rht12>Rht8>Rht13.The height dwarfization effect of promoted varieties and reserve lines was 9.8%and 9.3%,respectively.The inter‐node dwarfization effect value of section 4(base section 2)was 22.27%and 25.91%,respectively.【Con‐clusion】The most widely used dwarf genes in wheat in the Sichuan area are Rht-B1b,Rht-D1b and Rht8,and the applications of Rht5,Rht9 and Rht12 are increasing.

关 键 词：小麦 矮秆基因 株高 分子检测 

分 类 号：S512.1[农业科学—作物学]