不同生长速度的长吻鮠的消化、抗氧化、非特异性免疫酶和代谢酶活性的差异  

Differences in Digestive,Antioxidant,Nonspecific Immune and Metabolic Enzyme Activities of Longsnouted longirostris with Different Growth Rates

作　　者：贾垂攀 袁新程 刘永士 施永海 徐嘉波 蒋飞 邓平平 税春 JIA Chuipan;YUAN Xincheng;LIU Yongshi;SHI Yonghai;XU Jiabo;JIANG Fei;DENG Pingping;SHUI Chun(Shanghai Fisheries Research Institute/Shanghai Fisheries Technical Extension Station,Shanghai 200433,China)

机构地区：[1]上海市水产研究所/上海市水产技术推广站,上海200433

出　　处：《四川农业大学学报》2024年第6期1364-1370,共7页Journal of Sichuan Agricultural University

基　　金：上海市科技创新行动计划(22N11900600)。

摘　　要：【目的】旨在分析池塘养殖中长吻鮠生长速度存在差异的原因。【方法】采用池塘养殖的方式,将来自同源亲本的同龄同规格[体长(18.28±1.80)cm、体质量(94.63±27.01)g]的长吻鮠放到池塘中养殖229 d,然后拉网从一个池塘中获得950尾鱼,根据体质量从950尾中分别挑选前5%的最大和最小的个体作为生长快组和生长慢组的样本,每组样本为50尾,再从两组样本中随机各取15尾并随机分成3个平行,每个平行5尾,测量体长和体质量并测定胃、中肠和肝脏中的消化酶活性,肝脏和血清中的抗氧化酶、非特异性免疫酶和代谢酶的活性。【结果】生长快组平均体长为(26.77±0.45)cm,平均体质量为(277.84±17.80)g,生长慢组平均体长为(17.73±0.63)cm,平均体质量为(72.73±7.36)g,生长快组在体长与体质量上均显著大于生长慢组(P<0.05)。在消化酶中,生长快组的肝脏中淀粉酶、脂肪酶的活性显著高于生长慢组(P<0.05),胃中的淀粉酶、胃蛋白酶、脂肪酶、胰蛋白酶活性均显著高于生长慢组(P<0.05),肠道中的脂肪酶和胰蛋白酶活性显著高于生长慢组的长吻鮠(P<0.05)。在抗氧化酶中,生长快组的肝脏中的谷胱甘肽过氧化物酶、过氧化氢酶活性显著高于生长慢组(P<0.05),丙二醛含量显著低于生长慢组,血清中的谷胱甘肽过氧化物酶活性和丙二醛含量显著高于生长慢组,过氧化氢酶活性显著低于生长慢组(P<0.05)。在非特异性免疫酶和代谢酶中,生长快组的肝脏中的碱性磷酸酶和谷丙转氨酶活性显著高于生长慢组(P<0.05),血清中的酸性磷酸酶和谷丙转氨酶活性显著高于生长慢组(P<0.05)。【结论】试验条件下,生长快的长吻鮠的消化、抗氧化、非特异性免疫和代谢的酶在特定的器官或组织中活性高于生长慢的长吻鮠,说明生长快的长吻鮠的消化、抗氧化、免疫和代谢的能力强于生长慢的长吻鮠,为探究造成生长差异的�【Objective】This study aimed to analyze the factors contributing to growth rate discrepancies in Longsnouted longirostris reared in pond culture.【Method】Longsnouted longirostris of identical age and specifications[body length(18.28±1.80)cm,body weight(94.63±27.01)g]sourced from the same progenitors were pond-cultured for 229 days.Subsequently,950 fish were harvested from a single pond.The top 5%largest and smallest individuals based on body weight were selected from this pool as representatives of the fast-growth and slow-growth groups,respectively,each comprising 50 individuals.From these groups,15 individuals were randomly selected and further subdivided into 3 replicates of 5 individuals each.Measurements included body length,body weight,and the activity levels of digestive enzymes in liver,stomach,midgut,antioxidant enzymes and meta‐bolic enzymes in the liver and serum.【Result】The mean body length and weight of the fast-growth group[(26.77±0.45)cm,(277.84±17.80)g]was significantly greater than that of the slow-growth group[(17.73±0.63)cm,(72.73±7.36)g](P<0.05).In terms of digestive enzymes,the activi‐ties of amylase and lipase in the liver were significantly higher in the fast-growth group com‐pared to the slow-growth group(P<0.05).Additionally,activities of amylase,pepsin,lipase and tryp‐sin in the stomach were significantly higher in the fast-growth group(P<0.05).Lipase and tryp‐sin activities in the intestine were also significantly higher in the fast-growth group(P<0.05).Regarding antioxidant enzymes,glutathione peroxidase and catalase activities in the liver were significantly higher in the fast-growth group(P<0.05),while malondialdehyde content was sig‐nificantly lower compared to the slow-growth group.Glutathione peroxidase activity and malondial‐dehyde content in the serum were significantly higher in the fast-growth group,whereas catalase activity was significantly lower compared to the slow-growth group(P<0.05).For nonspecific immune and metabolic enzymes,alkaline phos

关 键 词：长吻鮠 消化酶 抗氧化酶 非特异性免疫酶 代谢酶 

分 类 号：S917.4[农业科学—水产科学]