piRNA及其生成相关基因在睾丸发育过程中的表达特征分析  

Expression Characterization Analysis of piRNA and Associated Genes during Testicular Development

作　　者：雷宇航 赵梦颖 王恺 周泰增 何晓菲 沈林園[1] 朱砺[1] 甘麦邻 LEI Yuhang;ZHAO Mengying;WANG Kai;ZHOU Taizeng;HE Xiaofei;SHEN Linyuan;ZHU Li;GAN Mailin(College of Animal Science and Technology,Sichuan Agricultural Univercity/Sichuan Provincial Key Laboratory of Animal Genetic Resources Exploration and Innovative Utilization/National Key Laboratory of Pig and Poultry Breeding/Key Laboratory of Livestock and Poultry Biomics,Ministry of Agriculture and Rural Affairs,Chengdu 611130,China)

机构地区：[1]四川农业大学动物科技学院/四川省畜禽遗传资源发掘与创新利用四川省重点实验室/猪禽种业全国重点实验室/农业农村部畜禽生物组学重点实验室,成都611130

出　　处：《四川农业大学学报》2024年第6期1371-1380,共10页Journal of Sichuan Agricultural University

基　　金：国家自然科学基金(32302689);四川省科技计划项目(24NSFSC1176、2021YFYZ0007、2021YFYZ0030);中国博士后科学基金(BX20230250、2023M732509);国家生猪产业技术体系(CARS-35)。

摘　　要：【目的】以不同发育时间点ICR小鼠为研究对象,旨在探究piRNA及其相关基因在小鼠睾丸中的表达规律,并为研究piRNA在睾丸发育和精子生成的相关调控机制提供理论依据。【方法】通过HE染色、qRT-PCR等技术手段,分析不同发育日龄小鼠睾丸组织的表型差异及piRNA和piRNA生成相关基因的表达水平,同时研究与piRNA相关的调控因子在睾丸发育过程中的动态变化和相关性分析。【结果】小鼠睾丸组织中的piRNA表达量呈现出明显的时序性变化,随着发育日龄的增长而逐渐增加,表明piRNA在小鼠睾丸发育过程中发挥着重要的调控作用。进一步分析发现,一些与piRNA相关的基因在不同发育阶段表达水平存在显著差异,提示这些基因可能参与了小鼠睾丸发育和精子生成的调控网络。【结论】揭示了piRNA在小鼠睾丸中的表达规律及其潜在的调控机制,为理解睾丸发育和精子生成过程中的分子调控提供了新的理论基础。【Objective】The purpose of this study was to investigate the expression patterns of piRNA and its related genes in the testes of ICR mice at different developmental time points,providing a theoretical basis for studying the regulatory mechanisms of piRNA in testicular development and sperm generation.【Method】Employing techniques such as HE staining and qRT-PCR,the phenotypic differences in testicular tissues of mice at different developmental ages and the expression levels of piRNA and piRNA production-related genes,were analyzed.At the same time,the dynamic changes and correlation analysis of regulatory factors related to piRNA during testicular development were investigated.【Result】The results demonstrate that the expression level of piRNA in murine testicular tissues exhibits significant temporal changes,gradually increasing with age,indicating an important regulatory role of piRNA in murine testicular development.Further analysis revealed significant differences in the expression levels of some genes related to piRNA at different developmental stages,suggesting that these genes may potential involve in the regulatory network of murine testicular development and sperm generation.【Conclusion】In summary,this study comprehensively elucidates the expression patterns of piRNA in murine testes and its potential regulatory mechanisms,providing a new theoretical foundation for understanding the mo‐lecular regulation of testicular development and sperm generation processes.

关 键 词：PIRNA 睾丸发育 精子生成 

分 类 号：Q418[生物学—生理学]