白头翁皂苷B4对幼犬二联苗免疫效果的影响  

The Impact of Anemoside B4 on the Immunization Effect of Diphtheria Vaccine in Young Canines

作　　者：罗皓 唐骏麒 唐春艳 罗羽欣 郑伟剑 曾致 巫晓峰[1] 陈欣 苏柘僮 沈留红[1] LUO Hao;TANG Junqi;TANG Chunyan;LUO Yuxin;ZHENG Weijian;ZENG Zhi;WU Xiaofeng;CHEN Xin;SU Zhetong;SHEN Liuhong(The Key Laboratory of Animal Disease and Human Health of Sichuan Province,The Animal Teaching Hospital,College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China;Bureau of Agriculture,Animal Husbandry,Rural Areas and Science and Technology of Yajiang County,Ganzi Tibetan Autonomous Prefecture,Yajiang 627450,Sichuan,China;Chengdu Xinhai Yinglu Biomedical Technology Co,Chengdu 610000,China)

机构地区：[1]四川农业大学动物医学院/动物疫病与人类健康四川省重点实验室/教学动物医院,成都611130 [2]雅江县农牧农村和科技局,四川雅江627450 [3]成都馨海英路生物医药科技有限公司,成都610000

出　　处：《四川农业大学学报》2024年第6期1399-1408,共10页Journal of Sichuan Agricultural University

基　　金：四川省科学技术厅重点研发项目(2024YFFK0146);四川农业大学学科建设双支计划;四川农业大学大学生创新创业训练项目(2321998237)。

摘　　要：【目的】旨在探究不同水平白头翁皂苷B4(Anemoside B4,AB4)对健康幼犬接种犬瘟热和犬细小二联苗后免疫应答反应的影响,以期为AB4在犬疫苗上的临床应用提供理论依据。【方法】选取6~8周龄临床检查健康的幼犬30只,随机分为空白组(Ⅰ组)、疫苗组(Ⅱ组)和疫苗+AB4组(分为低、中、高剂量AB4,记为Ⅲ、Ⅳ、Ⅴ组),6只/组,按照免疫程序,幼犬首次注射二联苗时记为D0,间隔14 d加免1次,共免疫3次,观察并记录D0、7、14、21、28、35和42各组幼犬体温、心率、呼吸频率和体重等临床指标,并检测外周血淋巴细胞增殖反应及抗原特异性抗体滴度。【结果】(1)AB4对幼犬体温、心率、呼吸频率等临床指标均无显著影响(P>0.05),体重和增重率各组间在同一时间也无显著差异(P>0.05),且临床未见其他明显异常。(2)不同剂量AB4均可显著提高外周血淋巴细胞增殖活性(P<0.05),且呈AB4剂量依赖性。(3)AB4可诱导CDV和CPV抗原特异性抗体滴度显著提高(P<0.05),且随AB4剂量增加而升高。【结论】AB4对幼犬免疫无明显临床副作用,可上调幼犬淋巴细胞增殖活性,诱导抗体分泌,增强二联苗对幼犬的免疫反应,具有作为幼犬疫苗佐剂潜力。【Objective】The purpose of this study was to investigate the effects of different levels of Ane‐moside B4(AB4)on the immune response of healthy young canines after inoculation with canine distem‐per and canine parvovirus,in order to provide a theoretical basis for the clinical application of AB4 in ca‐nine vaccines.【Method】In the experiment,thirty clinically healthy puppies aged 6 to 8 weeks were se‐lected and randomly divided into a blank group(GroupⅠ),a vaccine group(GroupⅡ),and a vaccine+AB4 group(divided into low-,medium-,and high-dose AB4 subgroups,denoted as GroupⅢ,GroupⅣ,and GroupⅤ),with 6 puppies in each group.According to the immunization schedule,the first injection of the combined vaccine for the puppies was recorded as D0,and a booster injection was given 14 days later,for a total of three immunizations.The clinical indicators such as body temperature,heart rate,respiratory rate,and body weight of each group of puppies at D0,D7,D14,D21,D28,D35,and D42 were observed and recorded,and the proliferation response of peripheral blood lymphocytes and the titer of antigen-specific antibodies were detected.【Result】①AB4 had no significant effect on body tempera‐ture,heart rate,respiratory rate and other clinical indicators of canines(P>0.05).There was no signifi‐cant difference in body weight and weight gain rate between the groups at the same time(P>0.05).②Dif‐ferent doses of AB4 could significantly increase the proliferation activity of peripheral blood lymphocytes in a dose-dependent manner(P<0.05).③AB4 could induce a significantly increase the titers of CDV and CPV antigen-specific antibodies(P<0.05),and the increase was dependent on the dose of AB4.【Conclusion】AB4 has no obvious clinical side effects on young canines'immunity.It can up-regulate the lymphocyte proliferation activity of canines,induce antibody secretion,and enhance the immune response of the bivalent vaccine to canines.It has the potential to be used as a vaccine adjuvant for young canines.

关 键 词：白头翁皂苷B4 幼犬 淋巴细胞增殖 疫苗免疫效果 抗原特异性抗体 

分 类 号：S854.5[农业科学—临床兽医学]