抗菌肽聚赖氨酸对胶原自组装的影响  

Effect of Antibacterialε-Polylysine on Collagen Self-assembly

作　　者：张晓霞[1,2] 李国英[1,2] ZHANG Xiaoxia;LI Guoying(Key Laboratory of Leather Chemistry and Engineering of Ministry of Education,Sichuan University,Chengdu 610065,China;National Engineering Laboratory for Clean Technology of Leather Manufacture,Sichuan University,Chengdu 610065,China)

机构地区：[1]四川大学皮革化学与工程教育部重点实验室,四川成都610065 [2]四川大学制革清洁技术国家工程实验室,四川成都610065

出　　处：《皮革科学与工程》2025年第1期8-14,共7页Leather Science and Engineering

基　　金：国家自然科学基金资助项目(22078206)。

摘　　要：将抗菌肽聚赖氨酸(ε-PL)引入胶原溶液,旨在探究生理条件下ε-PL对胶原(Col)自组装的影响。结果显示,阳离子性的ε-PL减缓了Col的自组装进程,组装产物网络结构趋于松散,胶原原纤维直径减小。当Col/ε-PL比例<1∶0.5时,仍可清晰地观察到Col自组装形成具有D周期的纤维,Col/ε-PL组装产物热稳定性与纯Col组装产物接近。并且,由于ε-PL的引入,Col/ε-PL组装产物表现出出色的抗菌活性。因此,适量引入抗菌肽ε-PL参与Col的自组装是一种构建抗菌性仿生胶原原纤维的有效策略。This study investigated the impact of antimicrobial peptide polylysine(ε-PL)on collagen(Col)self-assembly under physiological conditions.The results indicated that the cationicε-PL had a decelerating effect on the collagen selfassembly process,leading to a looser network structure and a decrease in the fibril diameter of collagen assemblies.When the Col/ε-PL ratio was below 1∶0.5,well-defined collagen fibrils with D-periodicity were observed,and the thermal stability of the Col/ε-PL assemblies was similar to that of pure collagen assemblies.Moreover,the introduction ofε-PL also enhanced the antimicrobial activity of the Col/ε-PL assemblies.Therefore,incorporating an optimal amount ofε-PL into collagen self-assembly represents a promising approach for developing antimicrobial biomimetic collagen fibrils.

关 键 词：胶原 聚赖氨酸 自组装 胶原原纤维 抗菌 

分 类 号：TS512[轻工技术与工程—皮革化学与工程]