1株野生羊肚菌的物种鉴定和栽培评价  

Species Identification and Cultivation Evaluation of a Wild Strain of Morchella spp.

作　　者：邓瑞民 李艳霞 张业胜 张晨[3] 陈凤仙 孙朝立 乌俊杰 DENG Ruimin;LI Yanxia;ZHANG Yesheng;ZHANG Chen;CHEN Fengxian;SUN Chaoli;WU Junjie(College of Agriculture and Biological Sciences,Dali University,Dali 671003,China;Shandong Junsheng Biotechnology Co.,Ltd.,Liaocheng 252000,China;School of Ecology and Environment,Northwestern Polytechnical University,Xi'an 710072,China;Shenxian Agriculture and Rural Bureau,Liaocheng 252400,China)

机构地区：[1]大理大学农学与生物科学学院,云南大理671003 [2]山东菌胜生物科技有限责任公司,山东聊城252000 [3]西北工业大学生态环境学院,陕西西安710072 [4]莘县农业农村局,山东聊城252400

出　　处：《中国食用菌》2024年第6期53-60,共8页Edible Fungi of China

基　　金：云南省应用基础研究青年项目(202001AU070021)。

摘　　要：对采自云南大理的1株野生羊肚菌菌株(Y88)进行形态学和分子系统学分析,确定该羊肚菌为六妹羊肚菌(Morchella sextelata)。经组织分离、纯化、交配型检测和活性继代测试,筛选出菌株Y88-2,经栽培后获得子实体。将收获的子实体再进行组织分离后复筛出菌株M1099-3、M1099-7和M1099-36。栽培试验结果显示M1099-3菌株产量最高,在樱桃园基地每667 m^(2)达264.26 kg。试验结果为羊肚菌产业发展提供了新的种质资源。A strain of wild Morchella spp.(Y88) was collected from Dali,Yunnan Province,and it was identified as Morchella sextelata by morphological and molecular systematics analysis.Strain Y88-2 was screened by tissue isolation,purification,mating type detection and activity passaging test,and further successful cultivated into fruit bodies.From the obtained fruit bodies,strains M1099-3,M1099-7 and M1099-36 were re-screened by tissue isolation.The results of cultivation experiment show that strain M1099-3 yielded up to 264.26 kg per 667 m~2 in the Yingtaoyuan base.The results provided new germplasm resources for the development of Morchella spp.industry.

关 键 词：六妹羊肚菌 分子系统学鉴定 交配型检测 人工栽培 

分 类 号：S646.9[农业科学—蔬菜学]