质谱流式细胞术在儿科疾病临床诊疗与研究中的应用  

Application of mass cytometry for clinical diagnosis,treatment and research in pediatric diseases

作　　者：沈红强[1,2] 田阳帆 舒强[1,2] Shen Hongqiang;Tian Yangfan;Shu Qiang(Binjiang Institute of Zhejiang University,Hangzhou 310053,China;Children's Hospital,Zhejiang University School of Medicine,National Clinical Research Center for Child Health,Hangzhou 310052,China)

机构地区：[1]浙江大学滨江研究院,杭州310053 [2]浙江大学医学院附属儿童医院国家儿童健康与疾病临床医学研究中心,杭州310052

出　　处：《中华检验医学杂志》2024年第12期1386-1390,共5页Chinese Journal of Laboratory Medicine

基　　金：中央引导地方科技发展资金(2023ZY1058)。

摘　　要：质谱流式细胞术(CyTOF)将传统流式细胞技术与质谱分析技术相结合, 其具有独特的金属标签标记、无需繁琐的补偿计算、高通量细胞分析以及多样化数据信息处理等优势, 可实现单细胞水平同时检测40多个参数, 其应用已涉及医学领域中的信号通路、生长发育、肿瘤微环境、自身免疫性疾病、感染、免疫治疗以及器官移植等方面。该文围绕CyTOF在儿科疾病临床诊疗与研究的最新应用进展进行阐述, 以期将CyTOF更好地应用于儿科疾病的诊治。Mass cytometry by time of flight(CyTOF)combines traditional flow cytometry with mass spectrometry,allowing for high-throughput,multiplexed measurements of up to 40 parameters.It offers advantages such as unique metal label tags,no need for compensation calculations,high throughput,and diversified data processing.CyTOF has been applied in many fields of medicine,including signaling pathways,growth and development,tumor microenvironments,autoimmune diseases,infections,immunotherapy,and organ transplantation.This article reviews recent progress in the application of CyTOF in the clinical diagnosis,treatment,and research of pediatric diseases to enhance its utility in diagnosis and treatment.

关 键 词：流式细胞术 质谱分析法 质谱流式细胞术 儿科学 免疫系统 肿瘤 

分 类 号：R72[医药卫生—儿科] R446[医药卫生—临床医学]