多参数流式细胞术精细化免疫细胞亚群参考区间的建立  

Establishment of reference intervals for refined immune cell subsets by multi-parameter flow cytometry

作　　者：谷春 王斐 李楠楠 陈美琦 侯雪筠[1] 费佳欣 毛润晗 安成[1] 王卉 李永哲[2] 刘贵建[1] 庞博[1] Gu Chun;Wang Fei;Li Nannan;Chen Meiqi;Hou Xueyun;Fei Jiaxin;Mao Runhan;An Cheng;Wang Hu;Li Yongzhe;Liu Guijian;Pang Bo(Clinical Laboratory,Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China;Department of Clinical Laboratory,State Key Laboratory of Complex Severe and Rare Diseases,Peking Union Medical College Hospital,Chinese Academy of Medical Science and Peking Union Medical College,Beijing 100730,China;Department of Clinical Laboratory,Hebei Yanda Lu Daopei Hospital,Langfang 065201,China)

机构地区：[1]中国中医科学院广安门医院检验科,北京100053 [2]中国医学科学院北京协和医院检验科,疑难重症罕见病国家重点实验室,北京100730 [3]河北燕达陆道培医院检验科,廊坊065201

出　　处：《中华检验医学杂志》2024年第12期1411-1418,共8页Chinese Journal of Laboratory Medicine

基　　金：横向课题(2021281)。

摘　　要：目的初步建立流式细胞术检测精细化免疫细胞亚群的参考区间。方法横断面研究。研究共纳入326名表观健康人, 按照年龄分为18~40岁和41~60岁组。空腹状态下采集外周静脉血, 参照既往专家共识方案进行流式细胞检测。依据CLSI EP28-A3c和中华人民共和国卫生行业标准WS/T 402-2024进行总体人群和不同年龄组的外周血精细化免疫细胞亚群的参考区间分析。结果检测结果主要分为T、B、NK、DC及单核细胞精细化亚群, 部分亚群的参考区间采用总人群的结果, 包括CD3^(+)亚群(56.4%~83.3%), CD4^(+)T_(EMRA)亚群(0.2%~11.6%), CD4^(+)TEM亚群(14.9%~52.8%), CD4^(+)CD28^(+)亚群(76.3%~99.9%), CD19^(+)CD5^(+)亚群(9.7%~45.8%), CD19^(+)CD27^(-)亚群(45.7%~90.0%), CD19^(+)CD27^(+)亚群(9.8%~54.0%), CD3^(+)CD16^(+)CD56^(+)亚群(1.3%~20.2%), CD3^(-)CD19^(-)CD20^(-)CD14^(-)CD56^(-)HLA-DR^(+)亚群(0.4%~2.0%), 单核细胞Mo1亚群CD14^(+)CD16^(-)(46.3%~94.6%), Mo2亚群CD14^(+)CD16^(+)(2.8%~49.7%)等。当不同年龄组间Z值>Z^(*)(截断值3.50)时, 提示这些亚群需根据年龄分组建立不同的参考区间。结论初步建立了多参数流式细胞术免疫细胞精细亚群的参考区间, 对于符合分组参考区间标准的亚群, 在临床应用及实验室验证时应充分考虑年龄因素。Objective:The aim of this study is to establish the reference interval of refined immune cell subsets by multi-parameter flow cytometry.Methods:In this cross sectional study,a total of 326 healthy participants were included and divided into two groups based on age:18-40 years old group and 41-60 years old group.Peripheral venous blood was collected in a fasting status.Flow cytometry tests were performed according to previous consensus article.The analysis of reference interval was conducted according to the documents of Clinical and Laboratory Standards Institute(CLSI)EP28-A3c and Health Industry Standards of the People′s Republic of China WS/T 402-2024.Results:The T,B,NK,DC and monocyte refined immune cell subsets applicable to the reference range of the general population mainly include:CD3^(+)(56.4%-83.3%),CD4^(+)TEMRRA(0.2%-11.6%),CD4^(+)TEM(14.9%-52.8%),CD4^(+)CD28^(+)(76.3%-99.9%),CD19^(+)CD5^(+)(9.7%-45.8%),CD19^(+)CD27^(-)(45.7%-90.0%),CD19^(+)CD27^(+)(9.8%-54.0%),CD3^(+)CD16^(+)CD56^(+)(1.3%-20.2%),CD3^(-)CD19^(-)CD20^(-)CD14^(-)CD56^(-)HLA-DR^(+)(0.4%-2.0%),Monocyte Mo1 subset CD14^(+)CD16^(-)(46.3%-94.6%),monocyte Mo2 subset CD14^(+)CD16^(+)(2.8%-49.7%),etc.When the Z-value between different age groups was higher than Z^(*)(3.50 cut-off value),the reference intervals of these subsets should be established independently according to age.Conclusions:In this study,the reference intervals of refined subsets of immune cells by multi-parameter flow cytometry has been preliminarily established.For those subgroups that meet the grouping criteria,age should be fully considered in clinical applications and laboratory validation.

关 键 词：流式细胞术 免疫细胞 参考区间 

分 类 号：R446[医药卫生—诊断学]