多参数流式细胞术在免疫衰老领域的应用进展  

The application progress of multiparameter flow cytometry in the field of immune senescence

作　　者：李楠楠 庞博[1] Li Nannan;Pang Bo(Clinical Laboratory,Guang'anmen hospital,China academy of Chinese medical sciences,Beijing 100053,China)

机构地区：[1]中国中医科学院广安门医院检验科,北京100053

出　　处：《中华检验医学杂志》2024年第12期1481-1486,共6页Chinese Journal of Laboratory Medicine

摘　　要：免疫衰老是人体衰老的重要组成部分, 与感染风险增加、肿瘤与心血管病等衰老相关疾病的发生发展密切相关。利用流式细胞术对免疫细胞的衰老标志物进行检测, 可评价不同类型免疫细胞的衰老状态。目前, 衰老研究多针对特定免疫细胞亚群, 全景描述不同类型免疫细胞衰老标志物的报道较少。免疫细胞种类繁多, 不同类型免疫细胞的衰老标志物也各不相同。中性粒细胞、单核细胞和树突状细胞等重要髓系细胞、固有淋巴细胞、获得性淋巴细胞、非常规T淋巴细胞等不同类型免疫细胞的衰老相关流式检测标志物与衰老的关系、数量变化及机制被重点分析, 整体展示了不同类型免疫细胞的常用衰老标志物。通过流式技术对这些标志物进行检测, 有利于描绘出人体的免疫衰老状态, 未来也可能有助于针对衰老疾病的干预和治疗进行评价。目前也存在免疫细胞精细亚群检测方案不统一、部分衰老标志物研究结果矛盾等问题, 仍有待进一步研究阐明, 以促进流式细胞检测在免疫衰老评估中的临床应用。Immunesenescence is an important component of human aging and is closely associated with age-related diseases such as cancer,cardiovascular disease,and increased risk of infection.Detection of immune cell senescence markers by flow cytometry provides a means to assess the senescence status of various immune cells.Current studies on immunesenescence are mostly focused on specific immune cell subpopulations with limited reports describing the senescence markers of different types of immune cells in the panorama.Given variety of immune cells,the aging markers differed across cell types.This paper provides a comprehensive analysis of the aging-related flow cytometry biomarkers of different types of immune cells such as neutrophils,macrophages and dendritic cells,congenital lymphocytes,acquired lymphocytes and unconventional T lymphocytes.The detection of these markers by flow cytometry is advantageous in depicting the immune senescence status of the human body and may also be useful in the future for evaluating interventions and therapies against age related diseases.However,challenges such as such as inconsistent detection protocols for refined subpopulations of immune cells and conflicting research results need further investigation to promote the clinical application of flow cytometry technology in the assessment of immune senescence.

关 键 词：流式细胞术 免疫衰老 衰老 

分 类 号：R446[医药卫生—诊断学]