长链非编码RNA生长阻滞特异性转录因子5、钙黏蛋白-11与绝经后2型糖尿病患者疏松性椎体压缩骨折风险的相关性研究  

Correlation of long non-coding RNA growth arrest-specific transcript 5 and cadherin-11 with the risk of osteoporotic vertebral compression fracture in postmenopausal patients with type 2 diabetes mellitus

作　　者：吴亚玲 贺佳 张苑 朱美霖 周媛昕 孙静[1] 韶龙格 包蕊 任丽珏[1] 张永红[1] 魏枫[1] Wu Yaling;He Jia;Zhang Yuan;Zhu Meilin;Zhou Yuanxin;Sun Jing;Shao Longge;Bao Rui;Ren Lijue;Zhang Yonghong;Wei Feng(Department of Endocrinology and Metabolism,First Affiliated Hospital of Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014010,China)

机构地区：[1]内蒙古科技大学包头医学院第一附属医院内分泌代谢科,包头014010

出　　处：《中华糖尿病杂志》2024年第12期1387-1393,共7页CHINESE JOURNAL OF DIABETES MELLITUS

基　　金：内蒙古自治区自然科学基金(2022MS08041);内蒙古自治区卫生健康委医疗卫生科技计划项目(202201410);2018年内蒙古第八批草原英才工程-滚动支持计划草原英才项目(2018(19))。

摘　　要：目的探讨外周血长链非编码RNA(lncRNA)生长阻滞特异性转录因子5(GAS5)、钙黏蛋白-11(Cad-11)表达水平与绝经后2型糖尿病(T2DM)患者疏松性椎体压缩骨折(OVCF)风险的相关性。方法选取2023年2至11月于内蒙古科技大学包头医学院第一附属医院内分泌科就诊并入组国家标准化代谢性疾病管理中心和脊柱外科住院治疗的133例绝经后T2DM患者,分为非骨质疏松(NOP)组(63例)、单纯骨质疏松(OP)组(31例)和OVCF组(39例),收集患者的体重、体重指数(BMI)等一般资料及生化指标,采用生物电阻抗法测量内脏脂肪面积(VFA)、皮下脂肪面积(SFA),计算内脏脂肪面积/皮下脂肪面积比值(VSR),采用双能X线吸收法测定腰椎(L_(1~4))骨密度(BMD)及相应T值,实时定量聚合酶链反应法检测外周血中lncRNAGAS5、Cad-11表达。多组间比较采用单因素方差分析、Kruskal-WallisH检验、χ^(2)检验,组间两两比较采用LSD-t检验、Mann-Whitney U检验。采用多因素logistic回归分析法分析绝经后T2DM患者OVCF的影响因素。结果与NOP组相比,OVCF组VFA升高,L_(1~4)BMD降低(P<0.05);与NOP、OP组比较,OVCF组患者BMI、SFA、体重降低,VSR升高(P<0.05)。LncRNAGAS5在NOP、OP、OVCF组中表达水平依次升高,Cad-11在3组中表达水平依次降低(P<0.05)。多因素logistic回归分析结果显示,lncRNAGAS5(OR=4.696,95%CI 1.149~19.187)、Cad-11(OR=0.180,95%CI 0.047~0.686)、BMI(OR=0.065,95%CI 0.011~0.372)是绝经后T2DM发生OVCF的影响因素。结论绝经后T2DM患者外周血中lncRNAGAS5高表达、Cad-11低表达与发生OVCF风险有关。Objective To investigate the correlation of long non-coding RNA(lncRNA)growth arrest-specific transcript 5(GAS5)and cadherin-11(Cad-11)expression levels in peripheral blood with the risk of osteoporotic vertebral compression fracture(OVCF)in postmenopausal patients with type 2 diabetes mellitus(T2DM).Methods A total of 133 postmenopausal T2DM patients who visited the Department of Endocrinology and enrolled in the National Standardised Metabolic Disease Management Centre and were hospitalised in the Department of Spine Surgery of the First Affiliated Hospital of Baotou Medical College of Inner Mongolia University of Science and Technology from February to November 2023 were selected and divided into non-osteoporosis(NOP)group(63 cases),osteoporosis(OP)group(31 cases)and OVCF group(39 cases).The weights,body mass index(BMI),and other general data and biochemical indexes of the patients were collected,and visceral fat area(VFA)and subcutaneous fat area(SFA)were measured by bioelectrical impedance method,and the ratio of visceral fat area to subcutaneous fat area(VSR)was calculated.The bone mineral density(BMD)of lumbar spine(L_(1-4))and the corresponding T-value were measured by dual-energy X-ray absorptiometry,and the expression of lncRNA GAS5 and Cad-11 in peripheral blood was detected by quantitative reverse transcription-polymerase chain reaction.One-way analysis of variance(ANOVA),Kruskal-Wallis H test,and χ^(2) test were used for comparison between multiple groups,and LSD-t test and Mann-Whitney U test were used for two-by-two comparison between two groups.Multivariate logistic regression analysis was used to analyze the influencing factors of OVCF in postmenopausal T2DM patients.Results Compared with the NOP group,VFA was increased and L_(1-4) BMD was decreased in the OVCF group,and BMI,SFA,and body weight were decreased and VSR was increased in the OVCF group compared with the NOP and OP groups(P<0.05).LncRNA GAS5 expression levels were sequentially increased in the NOP,OP,and OVCF groups,and Cad-11 was se

关 键 词：糖尿病 2型 骨折 压缩性 生长阻滞特异性转录因子5 钙黏蛋白11 

分 类 号：R587.2[医药卫生—内分泌] R683[医药卫生—内科学]