基于基因组重测序的沉香遗传多样性研究  

Genetic Diversity Studies on Aquilaria sinensis Based on Genome Resequencing

作　　者：苟志辉 陈国德 甄云楠 王鑫 陈彧 田蜜 GOU Zhihui;CHEN Guode;ZHEN Yunnan;WANG Xin;CHEN Yu;TIAN Mi(Hainan Academy of Forestry(Hainan Academy of Mangrove),Haikou,Hainan 571100,China;Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province,Haikou,Hainan 571100,China;Haikou City Wetland Protection and Management Center,Haikou,Hainan 571100,China)

机构地区：[1]海南省林业科学研究院(海南省红树林研究院),海南海口571100 [2]海南省热带林业资源监测与应用重点实验室,海南海口571100 [3]海口市湿地保护管理中心,海南海口571100

出　　处：《热带作物学报》2025年第1期51-58,共8页Chinese Journal of Tropical Crops

基　　金：海南省省属科研院所技术创新专项(No.KYYSLK2023-006,No.SQ-KY2022-0032)。

摘　　要：对种质资源信息的掌握是土沉香遗传改良的基础,而基因组重测序是种质资源遗传多样性分析的利器,然而目前学术界缺乏利用基因组重测序对土沉香遗传多样性的研究。本研究在前期收集得到的沉香种质资源基础上,采用基因组重测序,获得60份沉香共1284 Gb重测序数据。利用沉香基因组,共检测得到6 713 166个高质量变异位点,其中SNP位点6 284 344个,Indel位点428 658个。主成分分析、系统发育分析和遗传结构分析均表明,沉香可分为3个显著群体(Pop1、Pop2和Pop3),其中2个群体可分别进一步划分为多个亚群。在3个群体中均检测到群体选择信号,其中Pop3选择信号最多,而核苷酸多态性计算结果表明,Pop3的核苷酸多态性最低。本研究为探究沉香遗传资源多样性提供了有价值的信息,并为基于基因组的沉香遗传育种奠定理论基础。Knowledge on germplasm resource information is the foundation for the genetic improvement of Aquilaria sinensis,and genome resequencing is a powerful tool for analyzing the genetic diversity of germplasm resources.How-ever,there is no research on the genetic diversity of A.sinensis using genome resequencing.Based on the collection of A.sinensis germplasm resources in the early stages,this study utilized genome resequencing to obtain 1284 Gb of rese-quencing data from 60 samples of A.sinensis.Using the agarwood genome,6716166 high-quality variation sites were detected,including 6284344 SNP sites and 428658 Indel sites.Principal component analysis,phylogenetic analysis,and genetic structure analysis all indicated that A.sinensis could be divided into three distinct groups(Pop1,Pop2 and Pop3),with two of the groups further subdivided into multiple subgroups.Population selection signals were detected in all the groups,with Pop3 showing the highest frequency of selection signals.However,nucleotide polymorphism calcu-lations indicated that Pop3 had the lowest nucleotide polymorphism.This study would provide valuable information for exploring the genetic resource diversity of A.sinensis and lay a theoretical foundation for genome-based genetic breed-ing of A.sinensis.

关 键 词：沉香 基因组重测序 变异位点 遗传多样性 

分 类 号：S792.99[农业科学—林木遗传育种]