杂交兰双艺金龙根状茎增殖、分化及生根壮苗研究  

Rhizome Proliferation, Differentiation and Strong Seedlings of Cymbidium‘Shuangyi Jinlong’

作　　者：邓淑雯 吴悠 张华园 李世星 林子润 陈南川 傅春梅 饶小琼 吴沙沙[1,2] 翟俊文 DENG Shuwen;WU You;ZHANG Huayuan;LI Shixing;LIN Zirun;CHEN Nanchuan;FU Chunmei;RAO Xiaoqiong;WU Shasha;ZHAI Junwen(College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Key Laboratory of Orchidaceous Plant Conservation and Utilization,State Forestry and Grassland Administration,Fuzhou,Fujian 350002,China;Fujian Bainong Ecological Technology Co.Ltd.,Zhangzhou,Fujian 363599,China;Fujian Liancheng Orchid Co.,Ltd.,Longyan,Fujian 366211,China)

机构地区：[1]福建农林大学风景园林与艺术学院,福建福州350002 [2]兰科植物保育与利用国家林业和草原局重点实验室,福建福州350002 [3]福建百秾生态科技有限公司,福建漳州363599 [4]福建连城兰花股份有限公司,福建龙岩366211

出　　处：《热带作物学报》2025年第1期152-161,共10页Chinese Journal of Tropical Crops

基　　金：国家重点研发计划项目(No.2020YFD1000400);福建农林大学植物新资源开发与利用服务团队项目(No.11899170124);福建农林大学2021年度乡村振兴服务团队项目(No.11899170151)。

摘　　要：杂交兰双艺金龙(Cymbidium‘Shuangyi Jinlong’)是福建百秾生态科技有限公司从杂交兰栽培品种黄金龙的无性系变异单株中选育出的新品种,集花艺和叶艺于一身,发芽性好,易开花,抗性较强,有较高的市场需求。为提高杂交兰种苗繁殖效率,提升种苗品质,建立标准化生产体系,以双艺金龙的根状茎为材料,对其根状茎增殖与分化、生根壮苗、炼苗移栽等各阶段影响因素进行正交试验。结果表明:(1)适宜根状茎增殖生长的培养基是MS+1.00 mg/L NAA+0.10 mg/L 6-BA+20.00 g/L蔗糖+1.00 g/L活性炭+6.30 g/L琼脂+1.00 g/L蛋白胨,根状茎数量和鲜质量增殖系数分别为3.57和4.63。(2)适宜根状茎芽分化的培养基是1/2MS+1.50 mg/L NAA+0.90 mg/L TDZ+100.00 g/L香蕉泥+25.00 g/L蔗糖+6.30 g/L琼脂,芽分化率为97.78%,分化系数达6.87。(3)生根壮苗效果较好的培养基是1/2MS+1.00 mg/L IBA+100.00 g/L香蕉泥+0.17 g/L KH_(2)PO_(4)+1.00 g/L蛋白胨+30.00 g/L蔗糖+6.30 g/L琼脂+1.00 g/L活性炭,培养90 d植株平均生根数5条,根长5.40 cm,假鳞茎厚度4.35 mm,株高12.95 cm,每株苗鲜质量2.42 g。(4)炼苗21 d后选用水苔∶松树皮(V∶V=1∶1)为基质进行移栽,移栽60 d存活率为83.33%。本研究综合考虑各因素的影响效应,最终确定了各阶段的最佳培养基配方和炼苗移栽方案,为双艺金龙标准化生产体系的建立提供参考。Cymbidium‘Shuangyi Jinlong’is a new variety selected and bred from the clonal variation of the hybrid or-chid cultivar‘Golden dragon’by Fujian Bainong Ecological Technology Co.Ltd..It integrates flower and leaf art,has good germination,easy flowering and strong resistance,and has a high market demand.The rhizome and tissue culture seedlings of Cymbidium‘Shuangyi Jinlong’were used as experimental materials,and the orthogonal experiment was conducted to optimize the influencing factors of rhizome proliferation and differentiation,rooting and seedling growth,seedling cultivation and transplanting,in order to improve the seedling reproductive efficiency and seedling quality of hybrid orchid,and establish a standardized production system.The most suitable medium for rhizome proliferation and growth was MS medium supplemented with 1.00 mg/L NAA,0.10 mg/L 6-BA,20.00 g/L sucrose,1.00 g/L activated carbon,6.30 g/L agar,and 1.00 g/L peptone;the quantity value-added coefficient was determined to be 3.57 while the quality value-added coefficient was found to be 4.63.The optimal medium for rhizome differentiation was 1/2MS,1.50 mg/L NAA,0.90 mg/L TDZ,100.00 g/L banana homogenate,25.00 g/L sucrose 6.30g/L;the leaf bud differentia-tion rate reached up to 97.78%,with a differentiation coefficient of 6.87.The best-performing medium for rooting and seedlings strengthening was 1/2MS,l.00 mg/L IBA,100.00 g/L banana homogenate,0.17 g/L KH_(2)PO_(4),30.00 g/L su-crose,agar and l.00 g/L activated carbon.After 90 days of cultivation,the average number of roots per plant was five,with a root length of 5.40 cm,a pseudobulb thickness of 4.35 mm,a plant height of 12.95 cm,and a fresh quality of 2.42 g.The recommended method for transplanting tissue culture seedlings involves using water hyacinth∶pine bark(V∶V=1∶1)as the transplantation substrate after 21 days in vitro culture.The survival rate after 60 days post-transplantation reached 83.33%.Taking into account the impact of various factors,this study ultimately identifie

关 键 词：杂交兰 根状茎 增殖 分化 生根壮苗 

分 类 号：Q945.5[生物学—植物学] S682.31[农业科学—观赏园艺]