基于小RNA深度测序技术鉴定上海市郊区南瓜病毒种类  

Identification of pumpkin virus species in Shanghai suburbs based on small RNA deep sequencing technology

作　　者：袁瑗 奚丹丹 方草创 张红梅[1] 田守波[1] 吕铎 马坤[1] YUAN Yuan;XI Dandan;FANG Caochuang;ZHANG Hongmei;TIAN Shoubo;LÜDuo;MA Kun(Protected Horticultural Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai Key Lab of Protected Horticultural Technology,Shanghai 201106,China;Faculty of Food Science,Zhejiang Pharmaceutical University,Ningbo 315599,China)

机构地区：[1]上海市农业科学院设施园艺研究所,上海市设施园艺技术重点实验室,上海201106 [2]浙江药科职业大学食品学院,宁波315599

出　　处：《上海农业学报》2024年第6期81-88,共8页Acta Agriculturae Shanghai

基　　金：上海市科委重点攻关项目(2022DX1900100);上海市科技兴农技术创新项目(2021-02-08-00-12-F00765);国家自然科学基金项目(32302540)。

摘　　要：为鉴定上海市郊区秋季南瓜棚中的主要流行病毒病,于2020—2022年在奉贤区和青浦区采集21份南瓜叶片进行小RNA深度测序及PCR检测验证。结果表明:共鉴定出4种病毒,分别为新德里番茄曲叶病毒(ToLCNDV)、中国南瓜曲叶病毒(SLCCNV)、南瓜黄矮化失调病毒(CYSDV)和瓜类褪绿黄化病毒(CCYV)。其中,ToLCNDV和SLCCNV在不同年份和区域均被检测出,是上海市奉贤区和青浦区南瓜的主要流行病毒。此外,ToLCNDV和SLCCNV在黄瓜、甜瓜和西瓜中也被检测出。对来自15个国家64个ToLCNDV分离株的外壳蛋白(CP)序列进行系统进化树分析,结果显示:ToLCNDV的适应性进化与地理因素有关,ToLCNDV分离株被分为欧洲型和亚洲型两大类,中国内地的ToLCNDV分离株应来自中国台湾。To identify the main prevalent viral diseases in autumn pumpkin sheds in Shanghai suburbs,21 pumpkin leaf samples collected from Fengxian District and Qingpu District between 2020 and 2022 were validated by small RNA deep sequencing and PCR detection.The results showed that 4 viruses were identified,namely Tomato leaf curl New Delhi virus(ToLCNDV),Squash leaf curl China virus(SLCCNV),Cucurbit yellow stunting disorder virus(CYSDV),and Cucurbit chlorotic yellows virus(CCYV).Among them,ToLCNDV and SLCCNV had been detected in different years and regions,and were the main prevalent viruses in pumpkins in Fengxian District and Qingpu District of Shanghai.In addition,ToLCNDV and SLCCNV had also been detected in cucumbers,melons,and watermelons.Phylogenetic tree analysis of coat protein(CP)sequences of 64 ToLCNDV isolates from 15 countries showed that the adaptive evolution of ToLCNDV was related to geographical factors.ToLCNDV isolates were divided into 2 categories:Europe type and Asia type,and ToLCNDV isolates from China’s mainland should come from Taiwan,China.

关 键 词：小RNA深度测序 上海 南瓜病毒病 

分 类 号：S436.429[农业科学—农业昆虫与害虫防治]