ZIF-8固定化米曲霉脂肪酶并催化制备生物柴油  

Immobilization of Lipase from Aspergillus oryzae on ZIF-8 and Catalytic Preparation of Biodiesel

作　　者：吕珍珍 邢世友[2] 王超 吴慨凡[1,2] 李连华 苗长林[2] 罗文[2] LU Zhenzhen;XING Shiyou;WANG Chao;WU Kaifan;LI Lianhua;MIAO Changlin;LUO Wen(School of Energy Science and Engineering,University of Science and Technology of China,Guangzhou 510640,China;Guangzhou Institute of Energy Conversion,Chinese Academy of Sciences,Guangzhou 510640,China;School of Mechanical and Power Engineering,Shenyang University of Chemical Technology,Shenyang 110142,China)

机构地区：[1]中国科学技术大学能源科学与技术学院,广州510640 [2]中国科学院广州能源研究所,广州510640 [3]沈阳化工大学机械与动力工程学院,沈阳110142

出　　处：《新能源进展》2024年第6期656-663,共8页Advances in New and Renewable Energy

基　　金：国家重点研发计划项目(2022YFE0207100)。

摘　　要：生物柴油是一种绿色环保的生物液体燃料。提升酶法生产生物柴油的效率,寻找高效的脂肪酶固定化策略至关重要。金属有机框架具有孔隙率高、孔隙可调性好、易于功能化和修饰等独特的性能,是一种多孔结构的优良载体材料。以金属有机框架材料ZIF-8为载体,采用共沉淀法对米曲霉脂肪酶(AOL)进行原位包埋固定,制备纳米花结构的AOL@ZIF-8固定化酶。以该固定化酶AOL@ZIF-8、游离酶AOL及商业固定化酶Novozym 435作为催化剂,以大豆油为原料制备生物柴油,优化了生产工艺并比较了各催化剂的效果。在最优条件下,AOL@ZIF-8催化的生物柴油产率达到94.08%,其活性与重复使用性显著优于游离酶,与商业固定化酶Novozym435接近。Biodiesel is a sustainable and environmentally friendly bio-liquid fuel,and finding an efficient method for lipase immobilization to produce biodiesel enzymatically is a priority.Metal-organic frameworks,known for their high porosity,adjustable pore size,ease of functionalization,and modification,are excellent porous carrier materials.In this study,the metal-organic framework ZIF-8 was used as a carrier to immobilize lipase from Aspergillus oryzae(AOL)via co-precipitation,resulting in the formation of AOL@ZIF-8 with a nanoflower structure.Biodiesel was synthesized using soybean oil as raw material with AOL@ZIF-8,free AOL enzyme,and commercial immobilized enzyme Novozym 435 as catalysts.The production process was optimized,and the catalytic effects were subsequently compared.Under optimal conditions,the yield of biodiesel catalyzed by AOL@ZIF-8 reached 94.08%.Its activity and reusability were significantly better than the free enzyme and comparable to the commercial immobilized enzyme Novozym 435.

关 键 词：米曲霉脂肪酶 金属有机框架 ZIF-8 固定化 生物柴油 

分 类 号：TK6[动力工程及工程热物理—生物能] Q814.2[生物学—生物工程]