自组装π共轭聚合物微激光传感器的制备及在糖尿病患者呼出气体中丙酮检测上的应用  

Preparation of Self-Assembledπ-Conjugated Polymer Microlaser Sensor and Its Application in Detection of Acetone in Exhaled Gas of Diabetes Patients

作　　者：郭小慧[1] 樊滢玮[1] GUO Xiaohui;FAN Yingwei(Luohe Medical College,Luohe 462002,China)

机构地区：[1]漯河医学高等专科学校,漯河462002

出　　处：《理化检验（化学分册）》2024年第12期1240-1245,共6页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：漯河医学高等专科学校2020年度创新创业发展能力提升工程科研类项目(2020-LYZKYYB025)。

摘　　要：鉴于现有血糖检测方法多为有创测试,进行了题示研究。采用乳液-溶剂挥发法制备了聚[2-甲氧基-5-(2-乙基己氧基)-1,4-苯乙炔](MEH-PPV)微球,该微球具有完美圆形边界和超光滑表面,在330~380 nm紫外激发波长下发出均匀的红色荧光,发射峰位于601 nm和638 nm处。改变微球直径,在泵浦功率密度160 nJ·cm^(-2)下用飞秒激光激发微球,发现微球有效折射率(2.04)与MEH-PPV聚合物的固有折射率(1.85)比较吻合,说明激光调制是由球形回音壁腔共振引起的,有利于传感器感应共振波长处非常微小的变化。采用自行搭建气体传感器检测不同体积分数丙酮气体,丙酮气体体积分数在(2.0~4.0)×10^(-5)内与丙酮气体暴露前后激光波长变化量Δλs(暴露前激光波长λ0约621 nm)呈线性关系,检出限为9.0×10^(-8)(体积分数),且传感器对丙酮具有良好选择性;方法用于含2×10^(-6)(体积分数,下同)丙酮的模拟糖尿病患者呼出气体的分析,丙酮检出量为健康人呼出气体的4.05倍,2×10^(-6)丙酮气体的78%,说明研制的传感器可为糖尿病的无创诊断提供一种新方法。Given that most existing blood glucose detection methods are invasive tests,the study mentioned by the title was conducted.Poly[2-methoxy-5-(2-ethylhexoxy)-1,4-phenylacetylene](MEH-PPV)microspheres were prepared by the lotion-solvent evaporation method,which had perfect circular boundaries and super smooth surfaces,emitting uniform red fluorescence at 330-380 nm of UV excitation wavelength,with emission peaks at 601 nm and 638 nm.By changing the diameter of the microspheres excited by femtosecond laser at a pump power density of 160 nJ·cm^(-2),it was found that the effective refractive index(2.04)of the microspheres was consistent with the intrinsic refractive index(1.85)of the MEH-PPV polymer,indicating that laser modulation was caused by the resonance of the spherical whispering gallery cavity,which was beneficial for sensing very small changes at the resonance wavelength with the sensor.Using a self-built gas sensor to detect different volume fractions of acetone gas,the volume fraction of acetone gas within(2.0-4.0)×10^(-5) was linearly related to the changeΔλs in laser wavelength before and after exposure to acetone gas(the laser wavelengthλ0 before exposure was about 621 nm),with detection limit of 9.0×10^(-8)(volume fraction),and the sensor had good selectivity for acetone.The proposed method was used to analyze the exhaled gas of simulated diabetes patients containing 2×10^(-6)(volume fraction,the same below)acetone.The detected amount of acetone was 4.05 times of that in the 2×10^(-6) acetone gas,and 78%of that in the healthy individuals exhale gas.It was shown that the sensor could provide a new method for non-invasive diagnosis of diabetes mellitus.

关 键 词：共轭聚合物微球 微激光传感器 丙酮 糖尿病 无创检测 

分 类 号：O657.3[理学—分析化学]