一例新西兰白兔系统性淀粉样变性病理学诊断  

Pathological Diagnosis of Systemic Amyloidosis in a New Zealand White Rabbit

作　　者：林晴晴 戴锦龙 陈志森 郭健敏 杨威 LIN Qingqing;DAI Jinlong;CHEN Zhisen;GUO Jianmin;YANG Wei(Guangdong Lewwin Pharmaceutical Research Institute Co.,Ltd.,Guangzhou 510990,China;Guangzhou Bay Area Institute of Biomedicine,Guangzhou 510990,China;Guangzhou Provincial Key Laboratory of Drug Non-Clinical Evaluation and Research,Guangzhou 510990,China)

机构地区：[1]广东莱恩医药研究院有限公司,广州510990 [2]广州湾区生物医药研究院,广州510990 [3]广东省药物非临床评价与研究重点实验室,广州510990

出　　处：《实验动物与比较医学》2024年第6期695-699,共5页Laboratory Animal and Comparative Medicine

基　　金：广东省科技计划项目(科技创新平台类)“广东省药物非临床评价研究企业重点实验室”(2023B1212070029);广东省重大人才工程项目“'广东特支计划'科技创业领军人才-杨威”(2021TY060021)。

摘　　要：目的通过1例新西兰白兔系统性淀粉样变性的病理学诊断,为实验动物淀粉样变性的诊断提供参考。方法在一项为期6个月的重复滴眼给药毒性试验中,发现1只新西兰白兔的常规大体解剖检查异常,随后将其各脏器组织制作成石蜡切片,进行苏木素-伊红(hematoxylin-eosin,HE)染色和刚果红染色,于光学显微镜和偏振光显微镜下观察其病理组织学特征。结果大体解剖后肉眼形态观察见该例动物的脾脏偏大,肺脏颜色和质地改变。对各脏器组织进行HE染色后显示:脾脏组织结构被破坏,脾脏白髓周围被致密淀粉样沉积物以结节环的形式包围,白髓可见压力性萎缩;颌下淋巴结、肠系膜淋巴结以及回肠、圆小囊、蚓突、空肠、盲肠和直肠亦可见淀粉样蛋白沉积。刚果红染色显示,各受影响脏器组织内淀粉样蛋白呈鲑鱼粉色,在偏振光显微镜下呈现特殊的苹果绿双折光。结论该例新西兰白兔的各脏器组织病理特征符合系统性淀粉样变性的诊断特征。Objective To provide a reference for the diagnosis of amyloidosis in experimental animals through the pathological diagnosis of systemic amyloidosis in a case of a New Zealand white rabbit.Methods In a 6-month repeated ocular toxicity study,an abnormal finding was noted during the routine gross anatomical examination of one New Zealand white rabbit.Its organs were prepared as paraffin sections and stained with hematoxylin-eosin(HE)staining and Congo red staining.The histopathological features were observed under optical and polarized light microscopy.Results Gross anatomical examination of the animal revealed an enlarged spleen and changes in the color and texture of the lung.HE staining showed that the splenic tissue structure was destroyed,the white pulp of the spleen was surrounded by dense amyloid deposition in the form of nodular rings,along with pressure atrophy of the white pulp.Amyloid deposits were also observed in the submandibular lymph nodes,mesenteric lymph nodes,ileum,sacculus rotundus,vermiform appendix,jejunum,cecum,and rectum.Congo red staining showed that the amyloid deposition in the affected organs appeared salmon-pink,and exhibited characteristic apple green birefringence under polarized light microscopy.Conclusion The histopathological features of the New Zealand white rabbit are consistent with the diagnostic characteristics of systemic amyloidosis.

关 键 词：淀粉样变性 脾脏 病理形态学 新西兰白兔 

分 类 号：S852.31[农业科学—基础兽医学] Q95-33[农业科学—兽医学]