不同光质对两面针幼苗生长和主要活性成分的影响  

The Effects of Varying Light Qualities on the Growth and Main Active Components of Zanthoxylum nitidum Seedlings

作　　者：黄媛 朱艳霞[1] 庞丽华 郭晓云[1] HUANG Yuan;ZHU Yanxia;PANG Lihua;GUO Xiaoyun(National Center for TCM Inheritance and Innovation,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China;Guangxi University of Science and Technology,Liuzhou 545006,China)

机构地区：[1]广西壮族自治区药用植物园,国家中医药传承创新中心,广西南宁530023 [2]广西科技大学,广西柳州545006

出　　处：《照明工程学报》2024年第6期22-30,共9页China Illuminating Engineering Journal

基　　金：2022年广西重点研发项目“基于主要功效成分的两面针分子育种及产业链构建”(桂科AB22035068)。

摘　　要：为了考察不同光质对两面针幼苗生长发育的影响,以两面针幼苗为研究材料,以白光为对照(CK),采用LED光源处理,包括单一蓝光(B)、单一红光(R)和不同比例的红蓝光组合(T1:R∶B=2∶1;T2:R∶B=4∶1;T3:R∶B=6∶1;T4:R∶B=8∶1;T5:R∶B=10∶1),培养3个月后分析幼苗的农艺性状、根系形态、叶绿素含量、抗氧化酶活力、根系活力及生物碱含量等指标的差异。结果表明:光质对两面针幼苗生长影响显著,不同指标的影响程度不同。光质组合R∶B=4∶1(T2)培育的两面针幼苗地上部生长最优,其株高、茎粗分别是CK的1.7和1.4倍;光质组合R∶B=10∶1(T5)培育的两面针幼苗根生物量达到最大值,其根鲜重、根长、根投影面积、根表面积和根体积分别是CK的2.3、1.6、1.9、1.9和2.1倍;光质组合R∶B=10∶1(T5)培育的两面针幼苗超氧化物歧化酶(SOD)和过氧化物酶(POD)活性较对照都有显著提高,分别为对照的1.2和1.1倍;光质组合R∶B=8∶1(T4)和全蓝光(T6)培育的两面针幼苗总叶绿素含量显著高于对照,分别为对照的1.4和1.3倍;红蓝光促进两面针中生物碱的积累,其中红蓝组合R∶B=2∶1(T1)的生物碱含量是较对照的1.19倍。综合所有指标表现,红蓝光质组合R∶B=10∶1(T5)处理有效促进了两面针幼苗健壮生长,可作为工厂化育苗的人工光源。To investigate the effect of different light qualities on the growth and development of zanthoxylum nitidum seedlings,white light was used as the control(CK).LED light sources were applied,including single blue light(B),single red light(R),and different red-blue light combinations(T1∶R∶B=2∶1;T2:R∶B=4∶1;T3:R∶B=6∶1;T4:R∶B=8∶1;T5:R∶B=10∶1).After three months of cultivation,the differences in agronomic traits,root morphology,chlorophyll content,antioxidant enzyme activity,root vitality,and alkaloid content were analyzed.The results show that light quality significantly affects the growth of zanthoxylum nitidum seedlings,with varying degrees of impact on different indicators.The seedlings grown under the red-blue light combinations R∶B=4∶1(T2)exhibited the best above-ground growth,with plant height and stem diameter being 1.7 and 1.4 times that of the control(CK),respectively.The seedlings grown under the red-blue light combination R∶B=10∶1(T5)had the highest root biomass,with root fresh weight,root length,root projected area,root surface area,and root volume being 2.3,1.6,1.9,1.9 and 2.1 times that of the control,respectively.The seedlings grown under the R∶B=10∶1(T5)combination also showed significant increases in superoxide dismutase(SOD)and peroxidase(POD)activities,which were 1.2 and 1.1 times higher than the control,respectively.The seedlings grown under the R∶B=8∶1(T4)combination and full blue light(T6)had significantly higher total chlorophyll content compared to the control,with values being 1.4 and 1.3 times that of control,respectively.Red-blue light also promoted the accumulation of alkaloids in Niandranthes,with the alkaloids content of the R∶B=2∶1(T1)combination being 1.19 times that of the control.Considering all the indicators,the red-blue light combination R∶B=10∶1(T5)treatment effectively promoted the robust growth of Zanthoxylum nitidum seedlings,making it a suitable artificial light source for industrialized seedling cultivation.

关 键 词：两面针 育苗 生理特性 红蓝LDE灯 

分 类 号：S567[农业科学—中草药栽培]