深黑颜实蝇线粒体全基因组测定分析与系统发育  

Mitochondrial Whole Genome Assay and Phylogeny of Bactrocera(Zeugodacus)atrifacies(Dipitera,Tephritidae)

作　　者：黄振 郭琼霞 HUANG Zhen;GUO Qiongxia(Fuzhou Changle Airport Customs,Fuzhou 350209,Fujian,China;Fuzhou Customs Technical Center,Fuzhou 350001,Fujian,China)

机构地区：[1]福州长乐机场海关,福建长乐350209 [2]福州海关技术中心,福建福州350001

出　　处：《福建林业科技》2024年第4期8-16,42,共10页Journal of Fujian Forestry Science and Technology

基　　金：福建省自然科学基金项目(2011J01066、2012JO1061);福州海关科研项目(fk2022-09)。

摘　　要：基于Illumina HiSeq平台测序技术,对深黑颜实蝇(Bactrocera(Zeugodacus)atrifacies)进行线粒体基因组双端测序、基因拼接、注释和基因结构分析,测定深黑颜实蝇线粒体全基因组及结构特征,使用最大似然法(ML)构建系统发育树,分析其亲缘关系和系统发育。测定结果表明,深黑颜实蝇线粒体基因组序列总长度15866 bp(GenBank上的登录号为:MW892727),具37个基因(包含13个蛋白编码基因、22个tRNA基因、2个rRNA基因)和1个非编码控制基因。13个蛋白质编码基因22种氨基酸的密码子中,UUA(TTA(leucine))的使用频率N及相对密码子RSCU使用频率均最高,N(RSCU)为378(3.75);tRNA基因二级结构中,除苯丙氨酸(F)缺少假尿嘧啶(T)环和丝氨酸(S1)缺少二氢尿嘧啶DHU环外,其余20个tRNA基因均能折叠形成典型的三叶草二级结构图;线粒体tRNA基因中,共存在17处碱基对错配,错配的碱基对均为G-U;系统发育分析表明深黑颜实蝇与黑纹实蝇(B.(Z.)caudata)聚成一支,亲缘关系较近,属于镞果实蝇亚属。研究结果与传统形态学分类一致,为进一步的物种诊断、系统发育、进化生物学和防治研究提供参考。Based on the Illumina HiSeq platform sequencing technology,the mitochondrial genome double-end sequencing,gene splicing,annotation and gene structure analysis of Bactrocera(Zeugodacus)atrifacies were performed to determine the mitochondrial genome and structural characteristics of B.atrifacies.The phylogenetic tree was constructed using the maximum likelihood method(ML)to analyze its genetic relationship and phylogenetic development.The results showed that the total length of the mitochondrial genome sequence was 15866 bp(GenBank accession number:MW892727),with 37 genes(including 13 protein-coding genes,22 tRNA genes,2 rRNA genes)and 1 non-coding region.Among the codons of 22 amino acids of 13 protein-coding genes,UUA(TTA(leucine))had the highest frequency N and relative codon RSCU,with N(RSCU)of 378(3.75).In the secondary structure of tRNA gene,except for phenylalanine(F)lacking pseudouracil(T)ring and serine(S1)lacking dihydrouracil(DHU)ring,the other 20 tRNA genes could fold to form a typical clover secondary structure map.In the mitochondrial tRNA gene,there were 17 base pairs mismatched,and the mismatched base pairs were all G-U;phylogenetic analysis showed that B.atrifacies and B.(Z.)caudata were closely related and belonged to the subgenus Zeugodacus.The results were consistent with the traditional morphological classification,which provides a basis for further species diagnosis,phylogenetic,evolutionary biology and prevention research.

关 键 词：深黑颜实蝇 线粒体全基因组 序列分析 相对密码子使用频率 tRNA基因二级结构 

分 类 号：S763.41[农业科学—森林保护学]