湖北杂交枸杞浓浆饮料的营养品质及抗疲劳功效研究  

Nutritional quality and anti-fatigue effect of Hubei hybrid wolfberry thick pulp beverage

作　　者：邹志睿 朱和平 吴廉卿[1] 杨心彪 ZOU Zhirui;ZHU Heping;WU Lianqing;YANG Xinbiao(Wuhan Sports University,Wuhan 430079,China;Hubei Goji Zhen Liquor Co.,Ltd.,Jianshi 445300,China;College of Food Science and Technology,Huazhong Agricultural University/Key Laboratory of Environment Correlative Dietology,Ministry of Education,Wuhan 430070,China)

机构地区：[1]武汉体育学院,武汉430079 [2]湖北枸杞珍酒业有限公司,建始445300 [3]华中农业大学食品科学技术学院/环境食品学教育部重点实验室,武汉430070

出　　处：《华中农业大学学报》2024年第6期316-324,共9页Journal of Huazhong Agricultural University

基　　金：湖北省技术创新专项-民族专项(2017AKB074);中央预算内投资国家服务业发展引导资金项目(鄂发改投资[207]296号)。

摘　　要：为探究湖北杂交枸杞浓浆饮料的营养品质与缓解运动疲劳的功效,以湖北杂交枸杞为原料制备枸杞浓浆饮料,并与市售北方枸杞浓浆饮料对比,分析其理化成分特性和抗氧化能力;动物实验中,测定用不同剂量(4、8、12 mL/kg)枸杞浓浆饮料灌胃后小鼠的负重游泳力竭时间,以及运动后肝糖原(liver glycogen,LG)、肌糖原(muscle glycogen,MG)、血乳酸(blood lactic acid,BLA)、血尿素氮(blood urea nitrogen,BUN)、丙二醛(malondialdehyde,MDA)含量,超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽还原酶(gluathione reductase,GR)的活性,并与空白组的各项指标进行对比。结果显示,湖北杂交枸杞浓浆饮料含酚类(175.44μg/g)、黄酮(98.91μg/g)、蛋白质(1.90 mg/g)、甜菜碱(0.30 mg/mL)和Se(1.59μg/kg)等活性物质,其含量均高于市售枸杞浓浆饮料,并具有较好的抗氧化活性。湖北杂交枸杞浓浆饮料的中、高剂量组(8、12 mL/kg)可以有效延长小鼠的负重游泳时间,对缓解小鼠运动疲劳效果均有显著提高(P<0.05),分别是空白组的1.66和2.10倍,且高剂量组(12 mL/kg)是市售枸杞浓浆饮料组的1.32倍,差异显著(P<0.05);运动后湖北杂交枸杞浓浆饮料高剂量组小鼠的MG、LG含量相较空白组分别提高47.03%、116.22%,并且BLA、BUN、MDA含量均显著下降(P<0.05),SOD和GR活性均显著增加(P<0.05)。结果表明,湖北杂交枸杞浓浆饮料含有丰富的营养物质,具有良好的抗氧化活性和较好的抗疲劳效果,为湖北杂交枸杞功能性食品开发提供了新思路。Hubei hybrid wolfberry was used to study its nutritional quality and anti-fatigue effect and compare with that of the commercially available northern wolfberry as the raw material of the thick pulp beverage.The effects of Hubei hybrid wolfberry drink on relieving physical fatigue were investigated by analyzing its nutritional quality and determining the exhaustion time of swimming with a load in mice.In the animal experiments,the content of liver glycogen(LG),muscle glycogen(MG),blood lactic acid(BLA),blood urea nitrogen(BUN),malondialdehyde(MDA),superoxide dismutase(SOD),and glutathione reductase(GR)after exercise in mice after gavage of Hubei hybrid wolfberry thick pulp beverage at different dosage(4,8,12 mL/kg)were determined and compared with that of the blank group.The results showed that active substances including phenolics 175.44μg/g,flavonoids 98.91μg/g,proteins 1.90 mg/g,betaine 0.30 mg/mL,and Se 1.59μg/kg in Hubei hybrid wolfberry thick pulp beverage were higher than that of commercially available wolfberry thick pulp beverage,and had better antioxidant activity.The results of animal experiments showed that the medium-dosage and high-dosage group(daily gavage at 8 and 12 mL/kg)of Hubei hybrid wolfberry thick pulp beverage effectively prolonged the exhaustion time of swimming with a load in mice,and both of them significantly improved the anti-fatigue effect on mice(P<0.05),1.66 times and 2.10 times more than that in the blank group.The high-dosage group(daily gavage at 12 mL/kg)was 1.32 times higher than that of the commercially available commercially available wolfberry thick pulp beverage group,and the difference was significant(P<0.05).After exercise,the content of MG and LG of mice in the high dosage group of Hubei hybrid wolfberry thick pulp beverage increased by 47.03%and 116.22%compared to the blank group,and the content of BLA,BUN,and MDA significantly decreased(P<0.05),and the activity of SOD and GR significantly increased(P<0.05),indicating that Hubei hybrid wolfberry thick pulp beverage h

关 键 词：湖北杂交枸杞 抗疲劳 负重游泳 肝糖原 血尿素氮 运动饮料 

分 类 号：TS255.44[轻工技术与工程—农产品加工及贮藏工程]