Ginsenoside Rg1 promotes non-rapid eye movement sleep via inhibition of orexin neurons of the lateral hypothalamus and corticotropin releasing hormone neurons of the paraventricular hypothalamic nucleus  

作　　者：Yi-yuan Wang Yi Wu Ke-wei Yu Hong-yu Xie Yi Gui Chang-rui Chen Nian-hong Wang 

机构地区：[1]Department of Rehabilitation Medicine,Huashan Hospital,Fudan University,Shanghai 200040,China [2]National Center for Neurological Disorders,Shanghai 200040,China [3]National Clinical Research Center for Geriatric Diseases,Shanghai 200040,China [4]School of Rehabilitation Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China [5]Department of Pharmacology,School of Basic Medical Sciences,State Key Laboratory of Medical Neurobiology and Ministry of Education Frontiers Center for Brain Science,Institutes of Brain Science,Fudan University,Shanghai 200040,China

出　　处：《Journal of Integrative Medicine》2024年第6期719-728,共10页结合医学学报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(No.82174496,No.82374574);Shanghai Key Discipline of Traditional Chinese Medicine Construction Project(No.shzyyzdxk–2024113)。

摘　　要：Objective:This study investigates the sleep-modulating effects of ginsenoside Rg1(Rg1,C_(42)H_(72)O_(14)),a key bioactive component of ginseng,and elucidates its underlying mechanisms.Methods:C57BL/6J mice were intraperitoneally administered doses of Rg1 ranging from 12.5 to100 mg/kg.Sleep parameters were assessed to determine the average duration of each sleep stage by monitoring the electrical activity of the brain and muscles.Further,orexin neurons in the lateral hypothalamus(LH)and corticotropin-releasing hormone(CRH)neurons in the paraventricular hypothalamic nucleus(PVH)were ablated using viral vector surgery and electrode embedding.The excitability of LH^(orexin)and PVH^(CRH)neurons was evaluated through the measurement of cellular Finkel-Biskis-Jinkins murine osteosarcoma viral oncogene homolog(c-Fos)expression.Results:Rg1(12.5–100 mg/kg)augmented the duration of non-rapid eye movement(NREM)sleep phases,while reducing the duration of wakefulness,in a dose dependent manner.The reduced latency from wakefulness to NREM sleep indicates an accelerated sleep initiation time.We found that these sleep-promoting effects were weakened in the LH^(orexin)and PVH^(CRH)neuron ablation groups,and disappeared in the orexin and CRH double-ablation group.Decreased c-Fos protein expression in the LH and PVH confirmed that Rg1 promoted NREM sleep by inhibiting orexin and CRH neurons.Conclusion:Rg1 increases the duration of NREM sleep,underscoring the essential roles of LH^(orexin)and PVH^(CRH)neurons in facilitating the sleep-promoting effects of Rg1.Please cite this article as:Wang YY,Wu Y,Yu KW,Xie HY,Gui Y,Chen CR,Wang NH.Ginsenoside Rg1 promotes non-rapid eye movement sleep via inhibition of orexin neurons of the lateral hypothalamus and corticotropin-releasing hormone neurons of the paraventricular hypothalamic nucleus.J Integr Med.2024;22(6):719–728.

关 键 词：INSOMNIA Lateral hypothalamus Paraventricular hypothalamic nucleus Ginsenoside Rg1 Non-rapid eye movement 

分 类 号：R285.5[医药卫生—中药学]