A new bacterial concentration method for large-scale applications of biomineralization  

作　　者：Hanjiang Lai Xingzhi Ding Mingjuan Cui Junjie Zheng Jian Chu Zhibo Chen Jianwei Zhang 

机构地区：[1]Zijin School of Geology and Mining,Fuzhou University,Fuzhou,350108,China [2]Fujian-Taiwan Science and Technology Cooperation Base of Fujian Province on Intelligent Geo-Environmental Engineering,Fuzhou,350116,China [3]College of Civil Engineering,Fuzhou University,Fuzhou,350108,China [4]School of Civil Engineering,Wuhan University,Wuhan,430072,China [5]School of Civil and Environmental Engineering,Nanyang Technological University,Singapore [6]School of Civil Engineering and Architecture,Henan University,Kaifeng,475004,China

出　　处：《Journal of Rock Mechanics and Geotechnical Engineering》2024年第12期5109-5120,共12页岩石力学与岩土工程学报（英文）

基　　金：supports by the National Natural Science Foundation of China(Grant Nos.52108307 and 52178319);the National Natural Science Foundation of Fujian Province,China(Grant No.2022J05020).

摘　　要：Bacterial suspension is an essential component of microbially induced carbonate precipitation(MICP)-based biocement and a large-scale production is required for field applications.In this study,a new bacterial concentration method is proposed to enable high concentration bacterial suspension to be produced to facilitate field work.By adding low concentration calcium to bacterial suspension,flocs are formed and bacterial cells are adsorbed on the flocs to achieve bacterial concentration.Compared to the traditional bacterial concentration method using centrifugation and freezing-drying method,the proposed method can concentrate a large volume of bacterial suspension without using special equipment.The feasibility of this method is verified by bacterial concentration tests,solution tests and sand column treatment tests.The results of both the solution test and the sand column treatment test show that the bacterial suspension concentrated by the proposed method can be effectively used for soil biocementation.There is a threshold calcium concentration that allows a complete bacterial concentration for the proposed method,and this threshold calcium concentration tends to increase linearly with the optical density of the cell suspension at a wavelength of 600 nm(OD_(600)).

关 键 词：Biocement Biocementation Bacterial concentration Microbially induced carbonate precipitation (MICP) 

分 类 号：TU472[建筑科学—结构工程]