Effect of acid type on biomineralization of soil using crude soybean urease solution  

作　　者：Yajie Weng Hanjiang Lai Junjie Zheng Mingjuan Cui Yihang Chen Zhitao Xu Wensong Jiang Jianwei Zhang Yu Song 

机构地区：[1]School of Civil and Hydraulic Engineering,Huazhong University of Science and Technology,Wuhan,430074,China [2]Zijin School of Geology and Mining,Fuzhou University,Fuzhou,350108,China [3]School of Civil Engineering,Wuhan University,Wuhan,430072,China [4]College of Civil Engineering,Fuzhou University,Fuzhou,350116,China [5]School of Civil and Architecture Engineering,Henan University,Kaifeng,475004,China [6]School of Civil Engineering,Guilin University of Technology,Guilin,541006,China

出　　处：《Journal of Rock Mechanics and Geotechnical Engineering》2024年第12期5135-5146,共12页岩石力学与岩土工程学报（英文）

基　　金：support from the National Natural Science Foundation of China(NSFC)(Grant Nos.52338007 and 52178319);the Joint Fund of the Technical R&D Program of Henan Province(Grant No.225200810005);the technical assistance from the Analytical and Testing Center of Huazhong University of Science and Technology.

摘　　要：The one-phase-low-pH method is a simple,efficient,and user-friendly biogrouting technique that can effectively improve the biomineralization of enzyme-induced carbonate precipitation(EICP)using free urease enzyme.One of the most significant advantages of this method is its capacity to effectively delay calcium carbonate(CaCO3)precipitation by reducing the pH of the solution through the addition of acid.This prevents bioclogging during the biogrouting process and improves the biomineralization effect.However,the biomineralization of the one-phase-low-pH based EICP method may be influenced by the specific acid used.To investigate the impact of acid type on the one-phase-low-pH EICP method using crude soybean urease solution(CSUS),four types of acids,including hydrochloric acid(HCl),nitric acid(HNO_(3)),acetic acid(CH_(3)COOH),and lactic acid(C_(3)H_(6)O_(3)),were used to adjust the pH of CSUS.A series of macroscopic and microscopic experiments were conducted to evaluate the effect of acid type on the onephase-low-pH EICP method.The results indicate that the acid has an inhibition on the urease activity(UA)of CSUS.Among the acids tested,HNO_(3)exhibits the most pronounced inhibitory effect on the UA of CSUS,followed by HCl,and the least pronounced inhibitory effect for CH_(3)COOH and C3H6O_(3)under the same pH conditions.Meanwhile,CH_(3)COOH and C_(3)H_(6)O_(3)could provide a longer delay duration of CaCO_(3)precipitation than HNO_(3)and HCl.Therefore,the one-phase-low-pH EICP method based on CH_(3)COOH and C_(3)H_(6)O_(3)can significantly improve the effective biocementation depth compared to that based on HNO_(3)and HCl.Nevertheless,the different types of acids appear to have no obvious effect on the polymorph and crystalline of the precipitated CaCO_(3)crystals.

关 键 词：Enzyme-induced carbonate precipitation(EICP) One-phase-low-pH method Acid type Crude soybean urease solution(CSUS) BIOMINERALIZATION 

分 类 号：TU443[建筑科学—岩土工程]