苹果OPR基因家族鉴定及其在苹果绵蚜危害下的表达分析  

Identification of Apple OPR Gene Family and Analysis of Its Expression under the Damage of Wolly Apple Aphid,Eriosoma lanigerum

作　　者：高美瑞 滕子文 杜凌君 范银君 李津洋 万方浩 周洪旭 GAO Meirui;TENG Ziwen;DU Lingjun;FAN Yinjun;LI Jinyang;WAN Fanghao;ZHOU Hongxu(College of Plant Health&Medicine,Qingdao Agricultural University,Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management,Shandong Province Laboratory for Biological Invasions and Ecological Security,China-Australia Joint Institute of Agricultural and Environmental Health,Qingdao Shandong 266109,China;Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen Guangdong 518120,China)

机构地区：[1]青岛农业大学植物医学学院山东省环境友好型农业有害生物防治工程技术研究中心/山东省生物入侵与生态安全高校特色实验室/中澳农业与环境健康联合研究院,山东青岛266109 [2]中国农业科学院深圳农业基因组研究所,广东深圳518120

出　　处：《植物医学》2024年第6期53-63,共11页Plant Health and Medicine

基　　金：国家自然科学基金项目(32172395).

摘　　要：苹果绵蚜(Eriosoma lanigerum(Hausmann))是一种重要的果树害虫,不同苹果品种对苹果绵蚜抗性差异显著.茉莉酸对植物抗虫有重要的作用,12-氧-植物二烯酸还原酶(12-oxo-phytodienoic Acid Reductase,OPR)是控制茉莉酸合成的关键酶.利用不同品种在苹果绵蚜危害后的转录组学和蛋白质组学分析,研究茉莉酸代谢通路中OPR基因对苹果绵蚜的抗性作用.结果表明:通过本地Blast及功能域预测,共筛选到14个OPR基因.通过系统发育进化分析,MdOPRs可分为2类,MdOPR1,MdOPR3和MdOPR4与其他植物具有抗虫作用基因聚类在一起,属于OPRII亚类,其中MdOPR1和MdOPR4基因参与茉莉酸抗虫反应;其他11个基因属于OPR I亚类,不参与茉莉酸的抗虫反应.通过对苹果绵蚜危害不同品种苹果树转录组以及蛋白组数据分析,转录组筛选出5个有显著性差异的基因,从在不同品种中的表达量来看,2个基因在“红富士”中显著上升,在“小国光”和“新红星”中表达量显著下降或无显著性变化;根据蛋白组筛选出6个有显著性差异的基因,从在不同品种中的表达量来看,2个基因在3个品种中总体呈下降趋势,4个基因在3个品种中总体呈上升趋势.苹果树在被苹果绵蚜危害后,MdOPR1和MdOPR4表达量显著增加,表明MdOPR基因可能通过特定的表达模式响应苹果绵蚜对其的生物胁迫.Wolly apple aphid(Eriosoma lanigerum(Hausmann))is an important fruit pest,and the resistance of different apple varieties to E.lanigerum is significantly different.Jasmonic acid(JA)plays an important role in plant insect resistance.12-oxo-phytodienoic acid reductase(OPR)is a key enzyme that controls the synthesis of JA.In this paper,the transcriptomics and proteomics of different varieties after damage were used to study the resistance of OPR gene in jasmonic acid metabolism pathway against E.lanigerum.The results showed that a total of fourteen OPR genes were screened out by local Blast and functional domain prediction.According to phylogenetic analysis,MdOPRs can be divided into two classes.MdOPR1,MdOPR3 and MdOPR4 were clustered together with anti-herbivore genes from other plants,belonging to OPRⅡ subclass,in which,MdOPR1 and MdOPR4 genes were involved in anti-herbivore response of jasmonic acid.The other eleven genes belong to the OPRⅠsubclass and were not involved in the anti-herbivore response of jasmonic acid.By analyzing the transcriptome and proteome data of different varieties of apple trees affected by E.lanigerum,five genes with significant differences were screened out by transcriptome analysis.Regarding to the expression levels in different varieties,the expressions of two genes were significantly increased in‘Red Fuji’,and significantly decreased or had no significant changes in‘Ralls Genet’and‘Starkrimson’.According to the proteome analysis,six genes with significant differences were screened out.For the expression levels in different varieties,the expressions of two genes showed an overall downward trend in the three varieties,and four genes showed an overall upward trend in the three varieties.The expression levels of MdOPR1 and MdOPR4 in apple trees were significantly increased after E.lanigerum damage,suggesting that MdOPR gene may respond to biological stress by E.lanigerum through specific expression patterns.

关 键 词：苹果绵蚜 抗蚜机制 12-氧-植物二烯酸还原酶 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治]