分离富集磷酸化/糖基化蛋白质/肽的磁固相萃取新材料  

Magnetic Solid Phase Extraction Materials for Separation and Enrichment of Phosphoproteins/Peptides and Glycoproteins/Peptides

作　　者：鲁艳 张森 张锋[1] 葛鸿延 庄琬月 乔俊琴[1] 练鸿振[1] LU Yan;ZHANG Sen;ZHANG Feng;GE Hong-yan;ZHUANG Wan-yue;QIAO Jun-qin;LIAN Hong-zhen(State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry&Chemical Engineering and Center of Materials Analysis,Nanjing University,Nanjing 210023,China)

机构地区：[1]生命分析化学国家重点实验室,南京大学化学化工学院,南京大学现代分析中心,江苏南京210023

出　　处：《分析测试学报》2025年第1期1-11,共11页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(21874065,22176085,22304075);国家重点研发计划(2021YFF0600800)。

摘　　要：蛋白质磷酸化和糖基化是蛋白质两种主要的翻译后修饰过程。磷酸化和糖基化蛋白质在细胞的各个生命周期内起着关键的作用,对于临床疾病的早期诊断等也具有重要的意义。但由于它们在实际生物样品中含量低、基质干扰大,选择合适的方法对其进行分离富集成为蛋白质组学研究中决定性的步骤之一。磁固相萃取(MSPE)是以磁性纳米颗粒为吸附剂的固相萃取技术(SPE),较之常规SPE具有操作简单、环境友好、材料易于回收利用等优势,因而被广泛应用于磷酸化和糖基化蛋白/肽段等分析前的分离富集。该文对近5年文献中用于磷酸化和糖基化蛋白质分离富集的MSPE材料进行系统的归纳和评述,并对此类分离富集材料的未来发展方向进行了展望和探讨。Protein phosphorylation and glycation are two of the most prevalent post-translational mod⁃ification processes of proteins.Phosphoproteins and glycoproteins play an important role in the cell life cycle and are also of great significance for the early diagnosis of clinical diseases.However,due to the low content of them and large matrix interference in biological samples,finding an appropriate method for the separation and enrichment of phosphoproteins and glycoproteins is one of the decisive steps in proteomics research.Magnetic solid phase extraction(MSPE)is a solid phase extraction(SPE)technology with magnetic nanoparticles as adsorbent.Compared with conventional SPE,MSPE has the merits of simple operation and environmental friendliness,with its materials easy to re⁃cycle.Therefore MSPE has been widely applied for the separation and enrichment of phosphoproteins/peptides and glycoproteins/peptides before analysis.In this article,the MSPE materials used for sep⁃aration and enrichment of phosphoproteins and glycoproteins in recent five years are systematically summarized and reviewed,and the future development trend of such materials is prospected and discussed.

关 键 词：磷酸化蛋白质 糖基化蛋白质 磁固相萃取 磁性纳米颗粒 

分 类 号：O657[理学—分析化学] G353.11[理学—化学]