基于纸基微芯片的样本前处理方法研究进展  

Progress in Microfluidic Paper-based Analytical Devices of Sample Pre-preparation Methods

作　　者：马亚星 顾梦杰 李旭东 沈先涛 MA Ya-xing;GU Meng-jie;LI Xu-dong;SHEN Xian-tao(School of Public Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院公共卫生学院,湖北武汉430030

出　　处：《分析测试学报》2025年第1期91-98,共8页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(22276066)。

摘　　要：纸基微芯片(μPADs)是一种以纸质材料为基底的简易、便携式检测平台,在临床诊断、食品质量控制和环境监测等领域显示出极大的应用前景。作为快速检测平台,纸基微芯片除了需要对目标物进行快速定量,还要完成样本的前处理过程。科学家近年来开发了多种基于纸基微芯片的样本前处理方法,用于不同样本中目标物的分析。由于纸基具有强兼容性能,增强纸基芯片的样本前处理功能并将其与各种快速检测方法联用,能极大地扩展纸基微芯片的应用领域。该文对纸基微芯片的自驱动以及纸基微芯片自驱动与外驱动相结合的样本前处理方法进行了总结,概述了纸基微芯片在目标分析物提取、富集等前处理方面的研究进展,探讨了各种纸基微芯片前处理方法的优点和局限性。另外还展望了纸基微芯片的发展方向—除了不断提高样品前处理能力来扩展其在样本制备中的应用,还需要开发商业化的纸基微芯片来满足各类行业的检测需求。Microfluidic paper-based analytical devices(μPADs)are straightforward,portable test⁃ing platforms based on paper materials that demonstrate significant potential for applications in do⁃mains such as clinical diagnosis,food quality control,and environmental monitoring.As a rapid de⁃tection platform,μPADs need to complete the sample pre-treatment process in addition to the rapid quantification of the target.In recent years,scientists have developed a variety of sample pre-treat⁃ment methods based on paper-based chips for the analysis of targets in different samples.Due to the strong compatibility of paper,enhancing the sample pre-treatment function of paper-based chips and linking them with various rapid detection methods can greatly expand the application areas ofμPADs.This paper presents a summary of the sample pretreatment methods that including the paperbased chips with self-driving and the paper-based chips with external driving combined self-driving.It also outlines the research progress of paper-based chips in target analytes separation and enrich⁃ment,and discusses the advantages and limitations of various paper-based chips pre-treatment meth⁃ods.Furthermore,this paper looks at the direction ofμPADs,which need to be developed commer⁃cially to meet the testing needs of various industries,in addition to continuously improving sample preprocessing capabilities to expand their application in sample preparation.

关 键 词：快速分析 纸基微芯片 分离 驱动 前处理 

分 类 号：O652.6[理学—分析化学] TQ576.2[理学—化学]