五味子酯乙靶向PTGS1抑制子宫内膜癌细胞增殖和上皮间质转化  

Mechanism of Schisantherin B Inhibiting Proliferation of Uterine Corpus Endometrial Carcinoma Cells and Epithelial-mesenchymal Transition by Targeting PTGS1

作　　者：叶晶 李萍 赵金荣 韩慈 张欣 许园园 苏会玲 YE Jing;LI Ping;ZHAO Jinrong;HAN Ci;ZHANG Xin;XU Yuanyuan;SU Huiling(Department of Preventive Health Care,Affiliated Hospital of Non-Commissioned Officer School,Army Military Medical University,Shijiazhuang,050041,China;Department of Obstetrics and Gynecology,Affiliated Hospital of Non-Commissioned Officer School,Army Military Medical University,Shijiazhuang,050041,China;Department of Combat Rescue Medicine,Military Medical University,Shijiazhuang,050041,China)

机构地区：[1]陆军军医大学士官学校附属医院预防保健科,石家庄市050041 [2]陆军军医大学士官学校附属医院妇产科,石家庄市050041 [3]陆军军医大学士官学校战救医疗系,石家庄市050041

出　　处：《医学分子生物学杂志》2025年第1期23-28,共6页Journal of Medical Molecular Biology

基　　金：河北省卫生健康委科研基金(No.20191730);河北省2022年度医学科学研究课题(No.20221905)。

摘　　要：目的分析五味子酯乙靶向前列腺素内过氧化物合酶1(prostaglandin-endoperoxide synthase 1,PTGS1)抑制子宫内膜癌(uterine corpus endometrial carcinoma,UCEC)细胞增殖和上皮间质转化(epitheli-al-mesenchymal transition,EMT)的机制。方法TargetNet预测五味子酯乙的靶点PTGS1,TCGA数据库分析UCEC中高表达基因PTGS1与临床病理特征的关系。设置对照组、五味子酯乙组和sh-PTGS1组,检测各组细胞生存率、克隆细胞数和细胞EMT相关蛋白(E-cadherin、Vimentin、N-cadherin、Snail)表达情况。观察PTGS1对移植瘤体积、重量,和增殖指数(Ki67)表达的影响。构建稳定过表达PTGS1的Ishikawa和HEC108细胞,检测五味子酯乙对过表达PTGS1的Ishikawa和HEC108细胞存活情况、克隆细胞数和EMT蛋白表达的影响。结果与对照组比较,sh-PTGS1组和五味子酯乙组中细胞增殖和细胞克隆数以及PTGS1、Vimentin、N-cadherin、Snail表达明显下降,E-cadherin表达明显上升(P<0.05);sh-PTGS1组移植瘤模型中移植瘤组织体积、质量和Ki67表达水平均明显降低(P<0.05);PTGS1过表达组细胞中E-cad-herin明显下降,Vimentin、N-cadherin、Snail、PTGS1表达水平,Ishikawa和HEC108细胞活性和克隆细胞数明显上升(P<0.05)。与五味子酯乙组比较,五味子酯乙+PTGS1过表达组E-cadherin明显下降,Vim-entin、N-cadherin、Snail、PTGS1明显上升(P<0.05)。结论五味子酯乙可能通过靶向PTGS1,下调PTGS1表达水平,抑制UCEC增殖和EMT发生。Objective To analyze the mechanism of Schisantherin B inhibiting the proliferation of uterine corpus endometrial carcinoma(UCEC)cells and epithelial-mesenchymal transition(EMT)by targeting prostaglandin-endoperoxide synthase 1(PTGS1).Methods The target(PTGS1)of Schisantherin B was predicted by TargetNet.The relationship between the expression of PTGS1 and the clinicopathological characteristics of UCEC was analyzed by TCGA database.The control group,Schisantherin B group and sh-PTGS1 group were set up.The survival and colony formation abilities of cells,and the expression levels of EMT associated proteins were detected.The effect of PTGS1 on volume and weight of xenograft tumors,and the level of proliferation index(Ki67)were observed.Ishikawa and HEC108 cell lines with stable overexpression of PTGS1 were constructed.The effect of Schisantherin B on survival,colony formation abilities,and expression levels of EMT proteins of Ishikawa and HEC108 cells with PTGS1 overexpression were detected.Results Compared with those in the control group,the proliferation and numbers of cell colonies,and the expression levels of PTGS1,Vimentin,N-cadherin and Snail in the sh-PTGS1 group and the Schisantherin B group were significantly decreased,the expression level of E-cadherin in the above two groups was significantly increased;the volume and weight of xenograft tumor tissues,and the expression level of Ki67 in the sh-PTGS1 group were significantly decreased(P<0.05).However,the expression level of E-cadherin was significantly decreased in the PTGS1 overexpression group,the expression levels of PTGS1,Vimentin,N-cadherin,Snail,and the cell viabilities,and number of cell colonies of Ishikawa and HEC108 cells were significantly increased when compared with those in the control group(P<0.05).In addition,when compared with those in the Schisantherin B group,the expression level of E-cadherin was significantly decreased in the Schisandrin B+PTGS1 overexpression group,while the expression levels of PTGS1,Vimentin,N-cadherin,Snail were si

关 键 词：五味子酯乙 子宫内膜癌 细胞增殖 上皮间质转化 前列腺素内过氧化物合酶1 

分 类 号：R962.1[医药卫生—药理学]