两株植物根际促生菌对玉米土壤氧化亚氮排放的影响  

Effects of two plant growth promoting rhizobacterium strains on nitrous oxide emissions in maize soil

作　　者：胡椿 方晔 张欢欢 李林梅 高南 HU Chun;FANG Ye;ZHANG Huan-huan;LI Lin-mei;GAO Nan(College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing Jiangsu 211816)

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211816

出　　处：《中国土壤与肥料》2024年第11期57-69,共13页Soil and Fertilizer Sciences in China

基　　金：国家自然科学基金面上项目(31972503);南京工业大学大学生创新创业训练计划项目(2023DC1545)。

摘　　要：为探究植物根际促生菌(Plant growth promoting rhizobacteria,PGPR)对玉米生长和土壤氧化亚氮(N_(2)O)排放的影响,基于温室盆栽试验和16S r DNA扩增高通量测序技术,研究施氏假单胞菌(Pseudomonas stutzeri)NRCB010和贝莱斯芽孢杆菌(Bacillus velezensis)NRCB026对玉米幼苗生长和土壤N_(2)O排放的影响。与对照相比,接种NRCB010和NRCB026显著促进玉米幼苗的生长,干重分别增加41%和35%;减少土壤N_(2)O的累积排放量,分别降低54.6%和21.6%;在收获期,与对照相比,NRCB010处理土壤的细菌优势属中鞘氨醇单胞菌属(Sphingomonas)和黄杆菌属(Flavisolibacter)相对丰度分别增加12%和0.5%;NRCB026处理土壤的细菌优势属中鞘氨醇单胞菌属和黄杆菌属相对丰度分别增加11%和0.7%。FAPROTAX功能预测结果表明,NRCB010和NRCB026处理的土壤中反硝化和硝酸盐还原作用的微生物基因丰度高于对照。Pearson相关性和冗余分析进一步表明,pH是影响PGPR减少土壤N_(2)O排放的主要因素。NRCB010和NRCB026可能是通过增加玉米根际土壤反硝化微生物的丰度和活性实现减少土壤N_(2)O排放。研究结果为应用PGPR减少农田土壤N_(2)O排放提供一定的技术参考。To investigate the effects of plant growth promoting rhizobacteria(PGPR)on maize growth and soil nitrous oxide(N_(2)O)emission,the effects of control,Pseudomonas stutzeri NRCB010 and Bacillus velezensis NRCB026 on maize seedling growth and soil N_(2)O emission were compared based on the greenhouse potting experiment and 16S rDNA amplified high-throughput sequencing.Compared with the control,inoculation with NRCB010 and NRCB026 significantly promoted the growth of maize seedlings,increased dry weight by 41%and 35%,reduced cumulative soil N_(2)O emissions by 54.6%and 21.6%,respectively;at harvest,the relative abundance of Sphingomonas and Flavisolibacter in the dominant genera of soil bacteria in NRCB010 treatment increased by 12%and 0.5%,respectively,compared with the control;and the relative abundance of Sphingomonas and Flavisolibacter in NRCB026 treatment increased by 11%and 0.7%,respectively.FAPROTAX functional prediction results indicated that the abundance of microbial genes for soil denitrification and nitrate reduction was higher in NRCB010 and NRCB026 treatments than in the control group.Pearson correlation and redundancy analyses further indicated that pH was the main factor influencing PGPR to reduce soil N_(2)O emissions.NRCB010 and NRCB026 reduced soil N_(2)O emissions by increasing the abundance and activity of denitrifying microorganisms in maize rhizosphere soil.The results of this study provided a technical reference for the application of PGPR to reduce soil N_(2)O emissions in agricultural fields.

关 键 词：氧化亚氮 植物根际促生菌 微生物群落结构 施氏假单胞菌 贝莱斯芽孢杆菌 

分 类 号：S513[农业科学—作物学]