麻杏石甘汤对流感病毒肺炎小鼠肺组织NLRP3、IFITM3的影响  

Effects of Maxing Shigan Decoction on NLRP3 and IFITM3 in the lung tissues of Mice with Influenza Virus Pneumonia

作　　者：郝欧美[1] 李莹[2] 杨钦惠 张柯欣[2] 刘玉凤[3] HAO Ou-mei;LI Ying;YANG Qin-hui;ZHANG Ke-xin;LIU Yu-feng(Liaoning University of Traditional Chinese Medicine,Shenyang,110033,China;Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang,110847,China;Liaoning University of Traditional Chinese Medicine Affiliated Fourth Hospital,Shenyang,110100,China)

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110033 [2]辽宁中医药大学,辽宁沈阳110847 [3]辽宁中医药大学附属第四医院,辽宁沈阳110100

出　　处：《时珍国医国药》2024年第14期3113-3119,共7页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(82274580);辽宁省沈阳市科技计划项目(21-174-9-07)。

摘　　要：目的观察麻杏石甘汤对流感病毒肺炎小鼠肺组织中NLRP3、IFITM3的影响,探讨防治流感病毒感染的潜在机制。方法将72只雄性Balb/c小鼠随机分为4组,即麻杏石甘汤组、奥司他韦组、模型组和空白组。通过流感病毒滴鼻造模,治疗组给与麻杏石甘汤治疗,对照组给与奥司他韦颗粒治疗。分别在给药治疗第3、7天,各组分别取9只小鼠肺组织。应用Western Blot、RT-PCR方法,检测肺组织中NLRP3、IFITM3蛋白及mRNA的表达。结果给药3d后,与空白组相比,在模型组小鼠肺组织出现充血和水肿现象,伴有大量淋巴细胞和巨噬细胞浸润,肺泡结构明显破坏,肺泡塌陷甚至消失,且肺组织中NLRP3蛋白及mRNA表达量显著增高(P<0.01),IFITM3蛋白及mRNA表达量显著降低(P<0.01)。与模型组相比,麻杏石甘汤和奥司他韦均能调节小鼠肺组织中NLRP3及IFITM3的变化(P<0.01或P<0.05)。给药7d后,各药物组肺组织结构基本正常,奥司他韦组仍有少量炎性细胞浸润;麻杏石甘汤组和奥司他韦组肺组织形态接近空白组,肺泡腔轮廓清晰。与空白组相比,模型组肺组织中NLRP3蛋白及mRNA表达均有所增高(P<0.01),与模型组相比,麻杏石甘汤能显著降低肺组织中NLRP3的含量(P<0.01),同时增加IFITM3的表达(P<0.01),与奥司他韦组相比,麻杏石甘汤组肺组织中NLRP3、IFITM3的表达具有显著差异(P<0.05)。结论麻杏石甘汤可能通过抑制流感病毒肺炎小鼠肺组织中NLRP3的表达,同时上调IFITM3的表达,有效减轻肺部炎症,且效果优于奥司他韦,对流感病毒引起的肺损伤有一定的保护作用。Objective Study the effects of Maxing Shigan Decoction on the expression of NLRP3 and IFITM3 in the lung tissues of mice with influenza virus pneumonia,and explore the potential mechanisms for preventing and treating influenza virus infection.Methods-Randomly divide 72 male Balb/c mice into 4 groups:Maxing Shigan Decoction group,Oseltamivir group,model group,and blank group.The mice were intranasally infected with the influenza virus to establish the model.The treatment group was administered Maxing Shigan Decoction,while the control group was given Oseltamivir granules.On the 3rd and 7th days of treatment,9 mice from each group were selected for lung tissue collection.Western Blot and RT-PCR methods were used to detect the expression of NLRP3,IFITM3 proteins,and mRNA in the lung tissues.Results After 3 days of administration,compared with the control group,the mice in the model group showed signs of lung tissue congestion and oedema,accompanied by a large number of infiltrating lymphocytes and macrophages,significant damage to the alveolar structure,alveolar collapse or even disappearance,and a significant increase in the expression of NL-RP3 protein and mRNA in lung tissue(P<0.01),while the expression of IFITM3 protein and mRNA decreased significantly(P<0.01).Compared with the model group,Maxing Shigan Decoction and Oseltamivir could both regulate the changes in NLRP3 and IFITM3 in mouse lung tissue(P<0.01 or P<0.05).After 7 days of administration,the lung tissue structure in each drug group was basically normal,with a few inflammatory cells still infiltrating in the Oseltamivir group;the lung tissue morphology in the Maxing Shigan Decoction and the Oseltamivir group was similar to the control group,with clear alveolar contours.Compared with the control group,there was an increase in the expression of NLRP3 protein and mRNA in the lung tissue of the model group(P<0.01).In comparison with the model group,Maxing Shigan Decoction could significantly reduce the content of NLRP3 in lung tissue(P<0.01)while increasin

关 键 词：麻杏石甘汤 流感病毒 肺炎 NLRP3 IFITM3 

分 类 号：R285.5[医药卫生—中药学]