一种基于激发态分子内质子转移和聚集诱导发光性质的荧光探针在Cu^(2+)和谷胱甘肽检测中的应用  

Application of a Fluorescent Probe Based on Excited-State Intramolecular Proton Transfer and Aggregation-Induced Emission Properties for the Detection of Cu(Ⅱ)and Glutathione

作　　者：孙宇 李云妍 刘思阳 张岩 SUN Yu;LI Yunyan;LIU Siyang;ZHANG Yan(College of Chemistry,Chongqing Normal University,Chongqing 401331)

机构地区：[1]重庆师范大学化学学院,重庆401331

出　　处：《分析科学学报》2024年第6期620-626,共7页Journal of Analytical Science

基　　金：重庆市教委科技项目(KJQN202100502);大学生创新创业训练计划项目(202210637010)。

摘　　要：本文合成了对苯二亚氨基-2-苯(Phenyldiimide Salicylaldehyde,PDSA)分子,该分子有明显的聚集诱导发光性质和激发态分子内质子转移特性,并具有较大的斯托克斯位移(135 nm),利用该分子设计了一种高灵敏、高选择性荧光分析检测Cu^(2+)及谷胱甘肽(Glutathione,GSH)的方法。该分子在555 nm波长处的荧光随着Cu^(2+)加入而猝灭,以此实现Cu^(2+)检测。实验显示,当Cu^(2+)浓度在0.2~1.0μmol/L时,与荧光猝灭强度表现出很好的线性关系,检测限为14 nmol/L。PDSA与Cu^(2+)结合可作为复合探针(PDSA/Cu^(2+))用于GSH的荧光增强检测,且相比于其他干扰物,GSH具有更高的选择性和灵敏度,且在1.0~14.0μmol/L区间内具有良好的线性关系,检测限为179 nmol/L。此外,该方法在实际样品中也表现出良好的检测效果,在食品安全及疾病诊断等领域具有潜在的应用价值。A diphenylaniline-salicylic acid Schiff base compound(PDSA)had been synthesized.The molecule exhibits obvious aggregation-induced emission(AIE)and excited state intramolecular proton transfer(ESIPT)property and a large Stokes shift(135 nmol/L).A highly sensitive and selective fluorescence sensing method for Cu^(2+)and glutathione(GSH)was designed.The fluorescence of the molecule at 555 nm was quenched with the addition of Cu^(2+).When the concentration of Cu^(2+)ranged from 0.2μmol/L to 1.0μmol/L,the fluorescence intensity showed a good linear relationship with the concentration of Cu^(2+),and the detection limit was 14 nmol/L.PDSA and Cu^(2+)(PDSA/Cu^(2+))can be combined as a composite probe for the fluorescence enhancement detection of glutathione,and the composite probe has high selectivity and sensitivity.The composite probe has good linearity in the GSH concentration ranged from 1.0μmol/L to 14.0μmol/L,and the detection limit was about 179 nmol/L.In addition,the method also showed good test results in real samples.

关 键 词：席夫碱 聚集诱导发光 激发态分子内质子转移 铜离子 谷胱甘肽 

分 类 号：O657.39[理学—分析化学]