驴FBXL3基因在发情期和乏情期各组织表达谱差异及氨基酸特征分析  

Analysis on differential expression profiles and amino acid characteristics of donkey FBXL3 gene in tissues during estrus and anestrus stages

作　　者：刘亚星 冯玉龙 李健 李超程 张亚茹 王慧 王丽[1] 董红[2] 贾斌[1] 张永生[1] LIU Yaxing;FENG Yulong;LI Jian;LI Chaocheng;ZHANG Yaru;WANG Hui;WANG Li;DONG Hong;JIA Bin;ZHANG Yongsheng(College ofAnimal Science and Technoogy,ShiheziUniversity,Shihezi 832000,China;Instituteof Animal Husbandry,Xinjiang Academy of Animal Husbandry Sciences,Urumqi 830011,China)

机构地区：[1]石河子大学动物科技学院,新疆石河子832000 [2]新疆畜牧科学院畜牧研究所,乌鲁木齐830011

出　　处：《黑龙江畜牧兽医》2024年第23期116-120,129,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：兵团科技计划项目“多胎多羔肉羊高效繁育体系集成与示范”(KC2024CC007);新疆畜牧科学院项目“南疆四地州农区多胎多羔肉羊产业高效繁育技术研究与应用”(2021YED1600700)。

摘　　要：为了探究FBXL3基因在驴卵巢、卵泡液和乳腺的表达水平与驴发情期和乏情期之间的关系,试验以德州毛驴为研究对象,利用qPCR方法分析FBXL3基因在发情期和乏情期不同组织中的表达情况,利用生物信息学方法预测FBXL3蛋白质的理化性质、跨膜结构域、亚细胞定位、蛋白质相互作用情况、二级结构和三级结构。结果表明:FBXL3基因在发情期卵巢和卵泡液中的相对表达量极显著高于乏情期(P<0.01),乳腺中的相对表达量显著高于乏情期(P<0.05)。FBXL3蛋白有37个酶解位点,属于亲水性蛋白,无跨膜结构域,主要分布在线粒体上,为酸性、不稳定蛋白质;FBXL3蛋白中α-螺旋、β-转角、无规则卷曲和延伸链分别占49.30%、2.57%、35.98%和12.15%,与三级结构预测结果一致;SKP1、FBXL13、CRY1、CRY2、PER1、PER2等蛋白与FBXL3蛋白存在相互作用。说明FBXL3基因在发情期和乏情期的表达具有组织特异性。In order to investigate the expression levels of FBXL3 gene in donkey ovary,follicular fluid and mammary gland,and its relationship with donkey estrus and anestrus stages,in the experiment,Dezhou donkeys were used as the research object;qPCR method was used to analyze the expression of FBXL3 gene in different tissues during estrus and anestrus stages;bioinformatics methods were used to predict the physicochemical properties,transmembrane structural domains,subcellular localization,protein interactions,secondary and tertiary structures of the protein.The results showed that the difference in the expression of FBXL3 gene was highly significant(P<0.01)in ovarian and follicular fluid during estrus and anestrus stages,and significant(P<0.05)in mammary gland.FBXL3 protein had 37 enzymatic sites and was a hydrophilic protein;it had no transmembrane structural domains and was mainly distributed on mitochondria,and it was an acidic and unstable protein.Theα-helix,β-turn,irregularly coiled and extended chains in FBXL3 protein accounted for 49.30%,2.57%,35.98%and 12.15%,respectively,which was consistent with the tertiary structure prediction.Proteins such as SKP1,FBXL13,CRY1,CRY2,PER1 and PER2 interacted with FBXL3 protein.The results indicated that the expression of FBXL3 gene in estrus and anestrus stages was tissue-specific.

关 键 词：驴 FBXL3基因 表达分析 生物信息学 季节性发情 组织表达谱 

分 类 号：S822[农业科学—畜牧学]