血清中炎症因子与炎性衰老的相关性研究  

Correlation between inflammatory factors and inflamm-aging in human serum

作　　者：秦永生 苏俊 牟欣奕 石若湖 陈国兵 高利娟 QIN Yongsheng;SU Jun;MU Xinyi;SHI Ruohu;CHEN Guobing;GAO Lijuan(School of Environmental Engineering,Guangdong Polytechnic of Environment Protection Engineering,Foshan 528216,China;Ultrasound Medicine Department,the First Affiliated Hospital of Jinan University,Guangzhou 510632,China;School of Medicine,Jinan University,Guangzhou 510632,China)

机构地区：[1]广东环境保护工程职业学院环境工程学院,广东佛山528216 [2]暨南大学附属第一医院超声医学科,广东广州510632 [3]暨南大学基础医学与公共卫生学院,广东广州510632

出　　处：《广州医药》2024年第12期1407-1413,共7页Guangzhou Medical Journal

基　　金：国家自然科学基金(92169102)。

摘　　要：目的 探讨人外周血中炎症因子的表达与炎性衰老的相关性。方法 通过招募年轻和老年志愿者,检测外周血中炎症因子IL-6、IL-8、TNF-α和IL-1β的mRNA表达水平和蛋白水平。结果 显示老年个体组中IL-6,IL-8和TNF-α的mRNA表达水平高于年轻组,且蛋白水平呈现相同的趋势,比较差异均有统计学意义(P<0.05)。此外,通过热图分析了炎症因子mRNA和蛋白水平的相对表达谱,也发现相同的结果。结论 当年龄超过65岁以后,随着年龄的增长,体内的炎症因子表达水平会升高,这一结果具有成为炎性衰老生物标志物的潜力,对评估老年人的健康状况和疾病风险具有重要意义。Objective To explore the correlation between the expression of inflammatory factors in human peripheral blood and inflamm-aging.Methods In this experiment,the young and old volunteers were recruited to detect mRNA and protein levels of IL-6,IL-8,TNF-αand IL-1βin peripheral blood.Results The results indicated that mRNA expression levels of IL-6,TNF-αand IL-8 in the old individuals were higher than the young individuals,and the protein concentration followed the same trend,with acceptable P value suggesting a great statistically significant difference.In addition,the relative expression profiles of mRNA and protein concentrations of inflammatory cytokines were analyzed by heat map,and the same results were found.Conclusions It shows that the expression level of inflammatory cytokines up-regulates along with age goes on over 65,which has the potential of inflammatory biomarkers of aging.

关 键 词：炎性衰老 炎症因子 IL-6 IL-8 TNF-Α IL-1Β 生物标志物 

分 类 号：R339.38[医药卫生—人体生理学]