SLCO1B3基因甲基化水平与蛋壳颜色分析  

Methylation Level of SLCO1B3 Gene and Eggshell Color Analysis

作　　者：万火福 冯显钤 黄丽 吴亮 邱磊 WAN Huofu;FENG Xianqian;HUANG Li;WU Liang;QIU Lei(Guangxi Vocational University of Agricultural,Guangxi Nanning 530007)

机构地区：[1]广西农业职业技术大学,广西南宁530007

出　　处：《现代牧业》2024年第3期12-16,共5页Modern Animal Husbandry

基　　金：广西农业科技自筹项目(Z2022109);桂牧研科(2018-03)。

摘　　要：研究对162只健康的卢氏绿壳蛋鸡不同产蛋期内蛋壳颜色变化、SLCO1B3基因的表达水平及其甲基化状态、进行系统研究,并分析三者的相关性,以期揭示产蛋的颜色深浅变化的分子机制,为生产中蛋壳颜色一致性提供理论依据。试验采用NR色差计、qRT-PCR和重亚硫酸盐测序法对该批鸡在不同周龄(25~65周龄)所产蛋的蛋壳颜色、SLCO1B3基因相对表达量和基因启动子区甲基化水平进行测定。结果显示,随着产蛋周龄延长,蛋壳颜色逐渐变浅;SLCO1B3基因表达程度与蛋壳颜色显著正相关,而其甲基化程度与其表达量及蛋壳颜色显著负相关。研究表明,蛋壳颜色会随着卢氏绿壳蛋鸡产蛋时间的推移逐渐变淡,该改变与SLCO1B3基因表达量的减少以及基因甲基化水平的增加密切相关。This study systematically investigated the changes in eggshell color,the expression levels,and the methylation status of the SLCO1B3 gene in 162 healthy Lu’s green-shelled laying hens during different egg-laying periods,and analyzed the correlation between these three factors.The aim is to reveal the molecular mechanisms behind the variation in eggshell color depth and to provide theoretical support for achieving eggshell color consistency in production.Measurements were conducted using NR color difference meter,qRT-PCR,and bisulfite sequencing on eggs produced at various ages(25-65 weeks).Results showed that eggshell color gradually lightened with increasing laying age.Expression levels of the SLCO1B3 gene were significantly positively correlated with eggshell color,while gene methylation levels exhibited a significant negative correlation with both gene expression and eggshell color.The study indicates that as the laying age of Luoyang green-shelled hens increases,eggshell color lightens,a phenomenon closely associated with decreased SLCO1B3 gene expression and increased gene methylation levels.

关 键 词：SLCO1B3基因 SLCO1B3甲基化 蛋壳颜色 基因表达 

分 类 号：S834.2[农业科学—畜牧学]