HPLC-MS/MS法同时测定土茯苓七味散中12种成分的含量  

Simultaneous content determination of twelve constituents in Tufuling Qiwei Powder by HPLC-MS/MS

作　　者：胡玉霞 李君[1,2,3] 王跃武 吕晓丽[1,2] 张梦迪 李楠[1] 白图雅 常福厚 HU Yu-xia;LI Jun;WANG Yue-wu;LVXiao-li;ZHANG Meng-di;LI Nan;BAI Tu-ya;CHANG Fu-hou(College of Pharmacy,Inner Mongolia Medical University,Hohhot 010110,China;Engineering Research Center for New Drug Screening of Inner Mongolia Autonomous Region,Hohhot 010110,China;Center for New Drug Safety Evaluation and Research,Inner Mongolia Medical University,Hohhot 010110,China)

机构地区：[1]内蒙古医科大学药学院,内蒙古呼和浩特010110 [2]内蒙古自治区新药筛选工程研究中心,内蒙古呼和浩特010110 [3]内蒙古医科大学新药安全评价研究中心,内蒙古呼和浩特010110

出　　处：《中成药》2024年第12期3906-3910,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(82260733);内蒙古自治区自然科学基金(2023QN08016);内蒙古医科大学面上项目(YKD2022MS068);内蒙古医科大学2023年大学生科技创新“英才培育”项目(YCPY2023028);内蒙古医科大学2023年度大学生创新创业训练计划项目(X202310132040)。

摘　　要：目的 建立HPLC-MS/MS法同时测定土茯苓七味散中小檗碱、黄连碱、没食子酸、柯里拉京、栀子苷、川楝素、芦丁、绿原酸、木犀草素、木犀草苷、大黄素、落新妇苷的含量。方法 分析采用Shim-pack GIST-HP C_(18)色谱柱(2.1 mm×100 mm,3μm);流动相甲醇-水(含0.1%甲酸),梯度洗脱;体积流量0.25 mL/min;柱温35℃;电喷雾离子源;正负离子扫描;多反应监测模式。结果 12种成分在各自范围内线性关系良好(r≥0.999 0),平均加样回收率94.83%~104.42%,RSD 1.30%~4.88%。结论 该方法简便、快速、准确,可用于土茯苓七味散质量控制。AIM To establish an HPLC-MS/MS method for the simultaneous content determination of berberine,coptisine,gallic acid,corilagin,geniposide,toosendanin,rutinum,chlorogenic acid,luteolin,asiatica,emodin and astilbin in Tufuling Qiwei Powder.METHODS The analysis was performed on a 35 ℃thermostatic Shim-pack GIST-HP C_(18)column(2.1 mm×100 mm,3 μm),with the mobile phase comprising of methanol-water(containing 0.1%formic acid) flowing at 0.25 mL/min in a gradient elution manner,and electron spray ionization source was adopted in negative and positive ion detection with multiple reaction monitoring mode.RESULTS Twelve constituents showed good linear relationships within their own ranges(r≥0.999 0),whose average recoveries were 94.83%-104.42%with the RSDs of 1.30%-4.88%.CONCLUSION This simple,rapid and accurate method can be used for the quality control of Tufuling Qiwei Powder.

关 键 词：土茯苓七味散 化学成分 含量测定 HPLC-MS/MS 

分 类 号：R927.2[医药卫生—药学]